DETERMINATION OF CYCLODEXTRINS BY CAPILLARY LIQUID CHROMATOGRAPHY WITH INDIRECT CONDUCTIMETRIC DETECTION
CyclodextrinsÂ (CDs)Â containedÂ inÂ aÂ bottledÂ JapaneseÂ teaÂ andÂ aÂ wasabiÂ (JapaneseÂ horseradish)Â pasteÂ wereÂ determinedÂ byÂ capillaryÂ liquidÂ chromatographyÂ (LC)Â withÂ indirectÂ conductimetricÂ detectionÂ basedÂ onÂ dilutionÂ ofÂ theÂ mobileÂ phaseÂ dueÂ toÂ theÂ analytes.Â HighÂ electricalÂ conductivityÂ backgroundÂ wasÂ maintainedÂ byÂ additionÂ ofÂ 5Â mMÂ sodiumÂ nitrateÂ inÂ theÂ mobileÂ phase,Â andÂ theÂ analytesÂ wereÂ visualizedÂ byÂ decreasesÂ inÂ theÂ backgroundÂ whenÂ theyÂ eluted.Â TheÂ dynamicÂ reserve,Â definedÂ asÂ theÂ ratioÂ ofÂ theÂ backgroundÂ toÂ itsÂ noiseÂ level,Â achievedÂ underÂ theÂ presentÂ conditionsÂ wasÂ 1.7Ã—105,Â whichÂ wasÂ muchÂ largerÂ thanÂ thatÂ achievedÂ byÂ UVÂ absorptionÂ detection.Â ThisÂ meansÂ thatÂ indirectÂ conductimetricÂ detectionÂ gaveÂ betterÂ sensitivityÂ thanÂ indirectÂ UVÂ absorptionÂ detection.Â AÂ capacitivelyÂ coupledÂ contactlessÂ conductivityÂ detectorÂ wasÂ convenientÂ toÂ monitorÂ theÂ effluentsÂ fromÂ capillaryÂ columnsÂ withÂ minimumÂ extra-columnÂ bandÂ broadening.Â TheÂ signalsÂ asÂ negativeÂ peaksÂ wereÂ linearÂ toÂ theÂ concentrationÂ ofÂ theÂ analytes,Â andÂ theÂ concentrationÂ detectionÂ limitsÂ achievedÂ atÂ S/N=3Â wereÂ 0.02,Â 0.05Â andÂ 0.02%Â (w/v)Â forÂ Î±-,Â Î²-Â andÂ Î³-CD,Â respectively,Â correspondingÂ toÂ theÂ massÂ detectionÂ limitsÂ ofÂ 30-75Â ngÂ forÂ 0.15Â Î¼LÂ injection.Â TheÂ presentÂ detectionÂ methodÂ isÂ notÂ selectiveÂ butÂ universal,Â andÂ itÂ isÂ usefulÂ inÂ capillaryÂ LCÂ whenÂ analytesÂ haveÂ neitherÂ chromophoreÂ norÂ fluorophoreÂ groups.
Keywords:Â CapillaryÂ liquidÂ chromatography,Â IndirectÂ conductimetricÂ detection,Â CapacitivelyÂ coupledÂ contactlessÂ conductivityÂ detector,Â Cyclodextrins
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