MEKANISME INHIBISI ENZIM POLIFENOL OKSIDASE PADA SARI BUAH MARKISA DENGAN SISTEIN DAN ASAM ASKORBAT1

Authors

  • Elida Mardiah

DOI:

https://doi.org/10.25077/jrk.v4i2.126

Abstract

 

ABSTRACT

 

The mechanism of polyphenol oxidase enzyme inhibition was studied by isolation of enzyme from the passion fruite juice (Passiflora Sp). The extracted enzyme polyphenol oxidase has an optimum activity at pH 5,6 and temperature of 300 C using a pirogalol substrate. The pattern of inhibition of the enzyme polifenol oxsidase studied using cysteine and ascorbic acid. Cystein of 10 mM consentration can inhibit the enzyme polyphenol oxidase activity as 97.25%, ascorbic acid with the same concentration can inhibit the enzyme polyphenol oxsidace 96.5%. The pattern of inhibition of cysteine is more likely to be competitive, while non-competitive with ascorbic acid.

 

Keyword: polyphenol oxsidase, inhibition, cysteine, ascorbic acid

References

A. M. Mayer, Polyphenol Oxidases in Plant Recentprogress, Phitochemistry, 26, 11-20.

J. Klapp, A.H. Ricchard F.C., Inhibition Studies on Apple Polyphenol Oxidase, J.Food Chem, 38: 926 – 931, (1990).

Molnar, I. Perl, M. Friedman, Inhibitation of Browning bu Sulfur Amino Acid. 3. Apples and Potates, J. Agric. Food Chem, 38: 1652, (1990).

E.J. Lowrenco, Polyphenol Oxidase from Sweet Potato Purification and Properties, J.Agric Food Chem, 40: 2369–2373. (1992).

J.R. Chan, Passion Fruit in Tropical and Sub Tropical Fruit, John Willey and Sons Inc, (1978).

F. C. Richard Forged, and P.M. Goupy Cystein as inhibition of Enzymatic Browning .2. Kinetic Studies J.Agric Food Chem,41: 532- 536, (1993).

M. Y. Coseteng, C.Y. Lee, Changes in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning , J.Food Sci 52: 985-989, (1987).

S. Kremasha, Studies on Inhibitor Mushroom Polyphenol Oxidase using Chlorogenic Acid as Substrat., J.agric. Food Chem, 41: 526-531, (1993).

Eskin, Biochemistry of Foods, Academic Press, New York, 70-83, (1971).

Downloads

Published

2015-02-11

How to Cite

Mardiah, E. (2015). MEKANISME INHIBISI ENZIM POLIFENOL OKSIDASE PADA SARI BUAH MARKISA DENGAN SISTEIN DAN ASAM ASKORBAT1. Jurnal Riset Kimia, 4(2), 32. https://doi.org/10.25077/jrk.v4i2.126

Issue

Section

Articles

Citation Check