POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO

Authors

  • Urnemi -
  • Sumaryati Syukur
  • Endang Purwati
  • Sanusi Ibrahim
  • Jamsari -

DOI:

https://doi.org/10.25077/jrk.v1i2.149

Abstract


ABSTRACT

 

Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE. 

 

Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative 

                                                  

   

Author Biographies

Urnemi -

   

Sumaryati Syukur

   

Endang Purwati

   

Sanusi Ibrahim

   

Jamsari -

   

References

L. Seppo, T. Jauhiainen, T. Poussa, and R. Korpela, A fermented milk high in bioactive peptides has a blood pressure-lowering effect in hypertensive subjects. American Journal of Clinical Nutrition, 77, 326-330 (2004)

S. Taylor, Advances in Food and Nutrition Research, Vol. 50. Academic Press. ISBN 978-0-12-016450-9 (2004).

H.G. Fleet, The Microbial Ecology of Cocoa Bean Fermentation in Indonesia. Food Science and Technology School of Chemical Science University of New South Wales, Sidney. Australia (2003).

J. Serkedjieva, S. Danova, L. Ivanova, Antiinfluenza virus activity of a bacteriocin produced by Lactobacillus delbrueckii. Applied Biochemistry and Biotechnology 88: 285-298 (2000).

M.B. Wachsman, Enterococin CRL35 inhibits the last stage of HSV-1 and HSV-2 replication in vitro. Antiviral Research 58: 17-24 (2003)

Todorov, An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans. Int. J. of Antimicrobial Agents 25: 508-513 (2005).

S. Sumaryati dan E. Purwati, Peranan Pangan Probiotik Untuk Mikroba Patogen dan Kesehatan, Universitas Andalas Padang (2006).

J. J. Leisner, G. Rusul, B. W. Wee, H. C. Boo, and K. Mohammad, Microbiology of Chili Bo, A popular Malaysian food ingredient. Journal of Food Protection. 60:1235-1240 (1997).

De Man., J. C. Rogosa, M. E. Sharpe, A medium for the cultivation of lacto- bacilli. J. Appl.Bacteriol., 23, 130-135 (1960).

A. Mustopa, Colection Protocol Laboratorium Biotechnology and Virology Moleculer. Biotechnology Research Center. LIPI. Bogor (2007).

H. Holo, Nilssen, Nes IF, Lactococcin A, a new bacteriocin from Lactococcus lactis Subsp. cremoris: isolation and characterization of the protein and its gene. Journal of Bacteriology; 3879-87 (1991)

U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 277: 680- 685 (1970.

E. Purwati, Molecular Chacarcterization of Listeria spp. Isolated from Beef, Chicken and Fermented Fish in Malaysia (2003).

Kiran-kumar, P.V. Badarinath, P.M. Halami, Isolation of anti-listerial bacteriocin producing Lactococcus lactis CFR-B3 from Beans (Phaseolus vulgaris). The Internet Journal of Microbiology. Volume 6 Number 1 (2009).

Published

2015-02-12

How to Cite

-, U., Syukur, S., Purwati, E., Ibrahim, S., & -, J. (2015). POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO. Jurnal Riset Kimia, 1(2), 126. https://doi.org/10.25077/jrk.v1i2.149

Issue

Vol. 1 No. 2 (2008): March

Section

Articles

Citation Check

License

Please find the rights and licenses in Jurnal Riset Kimia (J. Ris. Kim). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.

1. License

The use the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License. 

2. Author(s)' Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights

Under the Creative Commons license, the journal permits users to copy, distribute, and display the material for any purpose. Users will also need to attribute authors and J. Ris. Kim on distributing works in the journal and other media of publications.

4. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

  • Copyright and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in own future works, including lectures and books,
  • The right to reproduce the article for own purposes,
  • The right to self-archive the article,
  • The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

5. Co-Authorship

If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. J. Ris. Kim will not be held liable for anything that may arise due to the author(s) internal dispute. J. Ris. Kim will only communicate with the corresponding author.