Validasi Metode MPM untuk Penentuan Kandungan Antioksidan dalam Sampel Herbal serta Perbandingannya dengan Metode PM, FRAP dan DPPH

Authors

  • Yefrida Yefrida Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia, Indonesia https://orcid.org/0000-0002-1212-2422
  • Hamzar Suyani Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia, Indonesia
  • Hermansyah Aziz Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia, Indonesia
  • Mai Efdi Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia

DOI:

https://doi.org/10.25077/jrk.v11i1.342

Keywords:

MPM method, antioxidant content, method comparison, method correlation

Abstract

The Modified Phenanthroline Method (MPM) has been validated for determination of antioxidant content in herb samples. Validation was done using Relative Standard Deviation (RSD) and percentage of recovery. The RSD and percentage of recovery for herb samples are 3.13% and 98.6%, respectively. Based on these values, MPM method is valid for determining antioxidant content in herb samples. T test shows no significant differences of antioxidant content using any of these methods, MPM, PM, FRAP or DPPH, at a 95% confidence level. MPM method shows a very strong correlation with PM and FRAP method.  While with DPPH and TPC shows is strong.

Author Biography

Yefrida Yefrida, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia

Scopus Profile:

https://www.scopus.com/authid/detail.uri?authorId=57191636458

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Published

2020-03-30

How to Cite

Yefrida, Y., Suyani, H., Aziz, H., & Efdi, M. (2020). Validasi Metode MPM untuk Penentuan Kandungan Antioksidan dalam Sampel Herbal serta Perbandingannya dengan Metode PM, FRAP dan DPPH. Jurnal Riset Kimia, 11(1), 24–34. https://doi.org/10.25077/jrk.v11i1.342

Issue

Vol. 11 No. 1 (2020): March

Section

Articles

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