Validasi Metode MPM untuk Penentuan Kandungan Antioksidan dalam Sampel Herbal serta Perbandingannya dengan Metode PM, FRAP dan DPPH

Yefrida Yefrida, Hamzar Suyani, Hermansyah Aziz, Mai Efdi


The Modified Phenanthroline Method (MPM) has been validated for determination of antioxidant content in herb samples. Validation was done using Relative Standard Deviation (RSD) and percentage of recovery. The RSD and percentage of recovery for herb samples are 3.13% and 98.6%, respectively. Based on these values, MPM method is valid for determining antioxidant content in herb samples. T test shows no significant differences of antioxidant content using any of these methods, MPM, PM, FRAP or DPPH, at a 95% confidence level. MPM method shows a very strong correlation with PM and FRAP method.  While with DPPH and TPC shows is strong.


MPM method; antioxidant content; method comparison; method correlation

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Kunwar, A. & Priyadarsini, K. I., Free radicals, oxidative stress and importance of antioxidants in human health. J Med Allied Sci, 1(2): 53–60 (2011).

Powers, S. K. & Jackson, M. J., Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production. Physiol. Rev., 88(4): 1243–1276 (2008).

Li, X. Y., Gilmour, P. S., Donaldson, K. & MacNee, W., Free radical activity and pro-inflammatory effects of particulate air pollution (PM10) in vivo and in vitro. Thorax, 51(12): 1216–1222 (1996).

P., L. J., Xi, L., Shengfeng, W., M.Y., L. M., D., B. G., R., M. M., R., C. F., et al., Reducing personal exposure to particulate air pollution improves cardiovascular health in patients with coronary heart disease. Environ. Health Perspect., 120(3): 367–372 (2012).

Ho, H., Cheng, M. & Chiu, D. T., Glucose-6-phosphate dehydrogenase–from oxidative stress to cellular functions and degenerative diseases. Redox Rep., 12(3): 109–118 (2007).

Xu, Y., Gu, Y. & Qian, S. Y., An advanced electron spin resonance (ESR) spin-trapping and LC/(ESR)/MS technique for the study of lipid peroxidation. Int. J. Mol. Sci., 13(11): 14648–14666 (2012).

Kinnula, V. L. & Crapo, J. D., Superoxide dismutases in malignant cells and human tumors. Free Radic. Biol. Med., 36(6): 718–744 (2004).

Singh, U. & Jialal, I., Oxidative stress and atherosclerosis. Pathophysiology, 13(3): 129–142 (2006).

Sas, K., Robotka, H., Toldi, J. & Vécsei, L., Mitochondria, metabolic disturbances, oxidative stress and the kynurenine system, with focus on neurodegenerative disorders. J. Neurol. Sci., 257(1–2): 221–239 (2007).

Smith, M. A., Rottkamp, C. A., Nunomura, A., Raina, A. K. & Perry, G., Oxidative stress in Alzheimer’s disease. Biochim. Biophys. Acta - Mol. Basis Dis., 1502(1): 139–144 (2000).

Bolton, J. L., Trush, M. A., Penning, T. M., Dryhurst, G. & Monks, T. J., Role of quinones in toxicology. Chem. Res. Toxicol., 13(3): 135–160 (2000).

Alam, M. N., Bristi, N. J. & Rafiquzzaman, M., Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm. J., 21(2): 143–152 (2013).

Bernhoft, A., Siem, H., Bjertness, E., Meltzer, M., Flaten, T. & Holmsen, E., Bioactive compounds in plants–benefits and risks for man and animals. The Norwegian Academy of Science and Letters, Oslo, (2010).

Ishrat, T., Hoda, M. N., Khan, M. B., Yousuf, S., Ahmad, M., Khan, M. M., Ahmad, A., et al., Amelioration of cognitive deficits and neurodegeneration by curcumin in rat model of sporadic dementia of Alzheimer’s type (SDAT). Eur. Neuropsychopharmacol., 19(9): 636–647 (2009).

Srivastava, J. K., Shankar, E. & Gupta, S., Chamomile: a herbal medicine of the past with a bright future. Mol. Med. Rep., 3(6): 895–901 (2010).

Jaradat, N. A., Al-Masri, M., Zaid, A. N., Hussein, F., Al-Rimawi, F., Mokh, A. A., Mokh, J. A., et al., Phytochemical, antimicrobial and antioxidant preliminary screening of a traditional Palestinian medicinal plant, Ononis pubescens L. Eur. J. Integr. Med., 14: 46–51 (2017).

Vyas, N., Tailang, M., Gavatia, N. P. & Gupta, B. K., Antioxidant potential of Psidium guajava Linn. Int. J. PharmTech Res., 2(1): 417–419 (2010).

Ojezele, O. J., Ojezele, M. O. & Adeosun, A. M., Comparative phytochemistry and antioxidant activities of water and ethanol extract of Annona muricata Linn Leaf, seed and fruit. Adv. Biol. Res. (Rennes)., 10(4): 230–235 (2016).

Yefrida., Suryani, H., Alif, A., Azis, H. & Efdi, M., Modification of phenanthroline method to determine antioxidant content in tropical fruits methanolic extract. Res. J. Chem. Environ., 22(4): 28–35 (2018).

Szydłowska-Czerniak, A., Dianoczki, C., Recseg, K., Karlovits, G. & Szłyk, E., Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta, 76(4): 899–905 (2008).

Molyneux, P., The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. sci. technol, 26(2): 211–219 (2004).

Horwitz, W., AOAC guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals. Gaithersburg, MD, USA: AOAC International, (2002).

Guilford, J., Fundamental statistics in psychology and education, 3e éd. (1956).

Chew, K. K., Khoo, M. Z., Ng, S. Y., Thoo, Y. Y., Aida, W. W. M. & Ho, C. W., Effect of ethanol concentration, extraction time and extraction temperature on the recovery of phenolic compounds and antioxidant capacity of Orthosiphon stamineus extracts. Int. Food Res. J., 18(4): 1427 (2011).

Naczk, M. & Shahidi, F., Extraction and analysis of phenolics in food. J. Chromatogr. A, 1054(1): 95–111 (2004).

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