Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

Authors

  • Sanusi Ibrahim Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang 25163, Indonesia, Indonesia
  • Suryati Suryati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang 25163, Indonesia
  • Enda Desriansyah Aziz Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang 25163, Indonesia, Indonesia

DOI:

https://doi.org/10.25077/jrk.v11i1.345

Keywords:

Gluta renghas L, secondary metabolites, cytotoxic, antibacterial

Abstract

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

References

Hyene, K., Tumbuhan berguna Indonesia. Jilid 3. Edisi I. Badan Libang Kehutanan, (1987).

Zuharah, W. F., Fadzly, N., Ali, Y., Zakaria, R., Juperi, S., Asyraf, M. & Dieng, H., Larvicidal efficacy screening of Anacardaciae crude extracts on the dengue hemorrhagic vector, Aedes aegypti. Trop. Biomed., 31(2): 297–304 (2014).

Supriatna, N. & Kelana, T., Informasi singkat benih Renghas (Gluta renghas L). Balai Pembenihan Tanaman Hutan Jawa dan Madura, 116 (2011).

Menon, S., Current uncertainties in assessing aerosol effects on climate. Annu. Rev. Environ. Resour., 29(1): 1–30 (2004).

Arbain, D., Survey fitokimia salah satu cara pendekatan, proyek HEDS. USAID. Universitas Andalas, (1995).

Lin, R. C. Y. & Whittow, G. C., Pharmacological activity of an aqueous extract of the leaves of the malayan rengas tree gluta renghas. Br. J. Pharmacol. Chemother., 15(3): 440–447 (1960).

Dransfield, J. & Corner, E. J. H., Wayside trees of Malaya. Kew Bull., 44(2): 372 (1989).

Copriady, J., Miharty. & Herdini., Gallokatekin: Senyawa flavonoid lainnya dari kulit batang Rengas (Gluta renghas Linn.). J. Natur Indones., 4(1): 1–6 (2002).

S, A. & Thamrin, M., Bahan tumbuhan sebagai pengendali hama ramah lingkungan. in Seminar Nasional Lahan Kering Dan Lahan Rawa, BPTP Kalimantan Selatan, (2002).

Zuharah, W. F., Ling, C. J., Zulkifly, N. & Fadzly, N., Toxicity and sub-lethal effect of endemic plants from family Anacardiaceae on oviposition behavior of Aedes albopictus. Asian Pac. J. Trop. Biomed., 5(8): 612–618 (2015).

Zuharah, W. F. & Yousaf, A., Assessment of Gluta renghas L. and Mangifera indica L. (Sapindales: Anacardiaceae) extracts on the sublethal effects of dengue vector. J. Asia. Pac. Entomol., 19(4): 1043–1051 (2016).

Yousaf, A. & Zuharah, W. F., Lethal response of the dengue vectors to the plant extracts from family Anacardiaceae. Asian Pac. J. Trop. Biomed., 5(10): 812–818 (2015).

Chomnawang, M. T., Surassmo, S., Nukoolkarn, V. S. & Gritsanapan, W., Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria. J. Ethnopharmacol., 101(1–3): 330–333 (2005).

Aiyelaagbe, O. O., Adeniyi, B. A., Fatunsin, O. F. & Arimah, B. D., In vitro antimicrobial activity and phytochemical analysis of Jatropha curcas roots. Int. J. Pharmacol., 3(1): 106–110 (2007).

J.B. Harborne., Metode fitokimia. Terbitan Kedua, Institut Teknologi Bandung, (1987).

Sasidharan, S., Darah, I. & Jain, K., In vivo and in vitro toxicity study of Gracilaria changii. Pharm. Biol., 46(6): 413–417 (2008).

Brook, G. ., Butel, J. . & Morse, S. A., Mikrobiologi Kedokteran (Medical microbiology),. Salemba Medika, (2005).

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M. & Yoga Latha, L., Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African J. Tradit. Complement. Altern. Med., 8(1): 1–10 (2011).

Cos, P., Vlietinck, A. J., Berghe, D. Vanden. & Maes, L., Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’. Journal of Ethnopharmacology, 106(3): 290–302 (2006).

Wahyu Ningdyah, A., Hairil Alimuddin, A. & Jayuska, A., Uji toksisitas dengan metode BSLT (Brine Shrimp Lethality Test) terhadap hasil fraksinasi ekstrak kulit buah tampoi (Baccaurea macrocarpa). JKK, 4(1): 75–83 (2015).

Meyer, B. N., Ferrigni, N. R. & Putnam, J. E., Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med., 45(1): 31–34 (1982).

Raineri, M., Histochemical localization of chitin in larvae of Artemia salina Leach (Phyllopoda). Bolletino di Zool., 48(2): 139–141 (1981).

Talaro, K., Foundations in microbiology, fourth edition. Harvard health letter / from Harvard Medical School, 34(10): Mc. Graw-Hill. Inc, (2009).

Bonang, G. & Koeswardono, E. S., Mikrobiologi Kedokteran Untuk Laboratorium dan Klinik. Gramedia, (1982).

Yudha, A. P., Senyawa antibakteri dari mikroalga Dunaliella sp pada umur panen yang berbeda. Institut Pertanian Bogor, (2008).

Downloads

Published

2020-03-30

How to Cite

Ibrahim, S., Suryati, S., & Aziz, E. D. (2020). Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L). Jurnal Riset Kimia, 11(1), 52–60. https://doi.org/10.25077/jrk.v11i1.345

Issue

Vol. 11 No. 1 (2020): March

Section

Articles

Citation Check

License

Please find the rights and licenses in Jurnal Riset Kimia (J. Ris. Kim). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.

1. License

The use the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License. 

2. Author(s)' Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights

Under the Creative Commons license, the journal permits users to copy, distribute, and display the material for any purpose. Users will also need to attribute authors and J. Ris. Kim on distributing works in the journal and other media of publications.

4. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

  • Copyright and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in own future works, including lectures and books,
  • The right to reproduce the article for own purposes,
  • The right to self-archive the article,
  • The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

5. Co-Authorship

If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. J. Ris. Kim will not be held liable for anything that may arise due to the author(s) internal dispute. J. Ris. Kim will only communicate with the corresponding author.