Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

Sanusi Ibrahim, Suryati Suryati, Enda Desriansyah Aziz

Abstract

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

Keywords

Gluta renghas L; secondary metabolites; cytotoxic; antibacterial

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References

Hyene, K., Tumbuhan berguna Indonesia. Jilid 3. Edisi I. Badan Libang Kehutanan, (1987).

Zuharah, W. F., Fadzly, N., Ali, Y., Zakaria, R., Juperi, S., Asyraf, M. & Dieng, H., Larvicidal efficacy screening of Anacardaciae crude extracts on the dengue hemorrhagic vector, Aedes aegypti. Trop. Biomed., 31(2): 297–304 (2014).

Supriatna, N. & Kelana, T., Informasi singkat benih Renghas (Gluta renghas L). Balai Pembenihan Tanaman Hutan Jawa dan Madura, 116 (2011).

Menon, S., Current uncertainties in assessing aerosol effects on climate. Annu. Rev. Environ. Resour., 29(1): 1–30 (2004).

Arbain, D., Survey fitokimia salah satu cara pendekatan, proyek HEDS. USAID. Universitas Andalas, (1995).

Lin, R. C. Y. & Whittow, G. C., Pharmacological activity of an aqueous extract of the leaves of the malayan rengas tree gluta renghas. Br. J. Pharmacol. Chemother., 15(3): 440–447 (1960).

Dransfield, J. & Corner, E. J. H., Wayside trees of Malaya. Kew Bull., 44(2): 372 (1989).

Copriady, J., Miharty. & Herdini., Gallokatekin: Senyawa flavonoid lainnya dari kulit batang Rengas (Gluta renghas Linn.). J. Natur Indones., 4(1): 1–6 (2002).

S, A. & Thamrin, M., Bahan tumbuhan sebagai pengendali hama ramah lingkungan. in Seminar Nasional Lahan Kering Dan Lahan Rawa, BPTP Kalimantan Selatan, (2002).

Zuharah, W. F., Ling, C. J., Zulkifly, N. & Fadzly, N., Toxicity and sub-lethal effect of endemic plants from family Anacardiaceae on oviposition behavior of Aedes albopictus. Asian Pac. J. Trop. Biomed., 5(8): 612–618 (2015).

Zuharah, W. F. & Yousaf, A., Assessment of Gluta renghas L. and Mangifera indica L. (Sapindales: Anacardiaceae) extracts on the sublethal effects of dengue vector. J. Asia. Pac. Entomol., 19(4): 1043–1051 (2016).

Yousaf, A. & Zuharah, W. F., Lethal response of the dengue vectors to the plant extracts from family Anacardiaceae. Asian Pac. J. Trop. Biomed., 5(10): 812–818 (2015).

Chomnawang, M. T., Surassmo, S., Nukoolkarn, V. S. & Gritsanapan, W., Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria. J. Ethnopharmacol., 101(1–3): 330–333 (2005).

Aiyelaagbe, O. O., Adeniyi, B. A., Fatunsin, O. F. & Arimah, B. D., In vitro antimicrobial activity and phytochemical analysis of Jatropha curcas roots. Int. J. Pharmacol., 3(1): 106–110 (2007).

J.B. Harborne., Metode fitokimia. Terbitan Kedua, Institut Teknologi Bandung, (1987).

Sasidharan, S., Darah, I. & Jain, K., In vivo and in vitro toxicity study of Gracilaria changii. Pharm. Biol., 46(6): 413–417 (2008).

Brook, G. ., Butel, J. . & Morse, S. A., Mikrobiologi Kedokteran (Medical microbiology),. Salemba Medika, (2005).

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M. & Yoga Latha, L., Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African J. Tradit. Complement. Altern. Med., 8(1): 1–10 (2011).

Cos, P., Vlietinck, A. J., Berghe, D. Vanden. & Maes, L., Anti-infective potential of natural products: How to develop a stronger in vitro ‘proof-of-concept’. Journal of Ethnopharmacology, 106(3): 290–302 (2006).

Wahyu Ningdyah, A., Hairil Alimuddin, A. & Jayuska, A., Uji toksisitas dengan metode BSLT (Brine Shrimp Lethality Test) terhadap hasil fraksinasi ekstrak kulit buah tampoi (Baccaurea macrocarpa). JKK, 4(1): 75–83 (2015).

Meyer, B. N., Ferrigni, N. R. & Putnam, J. E., Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med., 45(1): 31–34 (1982).

Raineri, M., Histochemical localization of chitin in larvae of Artemia salina Leach (Phyllopoda). Bolletino di Zool., 48(2): 139–141 (1981).

Talaro, K., Foundations in microbiology, fourth edition. Harvard health letter / from Harvard Medical School, 34(10): Mc. Graw-Hill. Inc, (2009).

Bonang, G. & Koeswardono, E. S., Mikrobiologi Kedokteran Untuk Laboratorium dan Klinik. Gramedia, (1982).

Yudha, A. P., Senyawa antibakteri dari mikroalga Dunaliella sp pada umur panen yang berbeda. Institut Pertanian Bogor, (2008).

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