Analysis of The Essential Oil from Lantana camara Leaves and Its Cytotoxic Potential Against T-47D Breast Cancer Cells

Authors

  • Suryati Suryati Organic Chemistry and Natural Products Laboratory, Department of Chemistry, Universitas Andalas, West Sumatra, Indonesia, Indonesia
  • Enda Desriansyah Aziz Organic Chemistry and Natural Products Laboratory, Department of Chemistry, Universitas Andalas, West Sumatra, Indonesia, Indonesia
  • Mai Efdi Organic Chemistry and Natural Products Laboratory, Department of Chemistry, Universitas Andalas, West Sumatra, Indonesia, Indonesia
  • Fatma Sri Wahyuni Cell Culture Laboratory, Faculty of Pharmacy, Universitas Andalas, West Sumatra, Indonesia, Indonesia
  • Dira Hefni Cell Culture Laboratory, Faculty of Pharmacy, Universitas Andalas, West Sumatra, Indonesia, Indonesia

DOI:

https://doi.org/10.25077/jrk.v12i1.364

Keywords:

Lantana camara, Cytotoxic, BSLT assay, MTT assay

Abstract

Lantana camara Linn is a family of Verbenaceae which grows wild and is widespread in various both tropical and sub-tropical countries. Isolation essential oil of the L. camara leaves extracted by hydrodistillation and were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results of GC-MS analysis show isocaryophyllene (14.39%), Ï-cymene (8.17 %), β-cubebene (7.8 %), α-pinene (7.64 %), and β-elemene (5.51 %) as the main compound. The cytotoxic activity of the isolated essential oil was highly toxic both to Artemia salina shrimp larvae (LC50 15.92 µg/mL) and to breast cancer cells T-47D (IC50 10.67 µM).

References

Ghisalberti, E. L., Lantana camara L. (Verbenaceae). Fitoterapia, 71(5): 467–486 (2000).

Ediruslan., Manjang, Y., Suryati. & Aziz, H., Structure elucidation of brine shrimp toxic compound from Lantana camara L. leaves. J. Chem. Pharm. Res., 7(12): 250–255 (2015).

Suryati, S., Malasari, Y., Efdi, M. & Mardiah, E., A Cytotoxic Compound from n-Hexane Fraction of Lantana camara Linn Leaves. Molekul, 14(1): 31 (2019).

Suryati., Mardiah, E., Efdi, M., Kartika, M. Z. & Sari, Y. M., Bioactivity of compounds isolated from the leaves of the Lantana camara Linn plant. J. Chinese Pharm. Sci., 28(5): 360–368 (2019).

Oluwadayo Sonibare, O. & Effiong, I., Antibacterial activity and cytotoxicity of essential oil of Lantana Camara L. leaves from Nigeria. African J. Biotechnol., 7(15): 2618–2620 (2008).

Syaiful., Zaeni, A., Suryani, R. & Hamsidi, J., Formulasi losio antinyamuk dengan zat aktif minyak atsiri Lantana camara Linn. J. Farm. dan Ilmu Kefarmasian Indones., 2(1): 2–5 (2015).

Oluwadayo Sonibare, O. & Effiong, I., Antibacterial activity and cytotoxicity of essential oil of Lantana Camara L. leaves from Nigeria. African J. Biotechnol., 7(15): 2618–2620 (2008).

Deena, M. J. & Thoppil, J. E., Antimicrobial activity of the essential oil of Lantana camara. Fitoterapia, 71(4): 453–455 (2000).

Zandi-Sohani, N., Hojjati, M. & Carbonell-Barrachina, Ã. A., Bioactivity of Lantana camara L. essential oil against Callosobruchus maculatus (Fabricius). Chil. J. Agric. Res., 72(4): 502–506 (2012).

Verdeguer, M., Blázquez, M. A. & Boira, H., Phytotoxic effects of Lantana camara, Eucalyptus camaldulensis and Eriocephalus africanus essential oils in weeds of Mediterranean summer crops. Biochem. Syst. Ecol., 37(4): 362–369 (2009).

Zoubiri, S. & Baaliouamer, A., GC and GC/MS analyses of the Algerian Lantana camara leaf essential oil: Effect against Sitophilus granarius adults. J. Saudi Chem. Soc., 16(3): 291–297 (2012).

Dua, V. K., Pandey, A. C. & Dash, A. P., Adulticidal activity of essential oil of Lantana camara leaves against mosquitoes. Indian J. Med. Res., 131(3): 434–9 (2010).

Khan, M., Srivastava, S. K., Syamasundar, K. V., Singh, M. & Naqvi, A. A., Chemical composition of leaf and flower essential oil of Lantana camara from India. Flavour Fragr. J., 17(1): 75–77 (2002).

Meyer, B., Ferrigni, N., Putnam, J., Jacobsen, L., Nichols, D. & McLaughlin, J., Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Planta Med., 45(05): 31–34 (1982).

Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65(1–2): 55–63 (1983).

Segun, P. A., Ogbole, O. O., Ismail, F. M. D., Nahar, L., Evans, A. R., Ajaiyeoba, E. O. & Sarker, S. D., Bioassay-guided isolation and structure elucidation of cytotoxic stilbenes and flavonols from the leaves of Macaranga barteri. Fitoterapia, 134: 151–157 (2019).

Machado, R. R. P., Valente Júnior, W., Lesche, B., Coimbra, E. S., Souza, N. B. de., Abramo, C., Soares, G. L. G., et al., Essential oil from leaves of Lantana camara: a potential source of medicine against leishmaniasis. Rev. Bras. Farmacogn., 22(5): 1011–1017 (2012).

Nea, F., Tanoh, E. A., Wognin, E. L., Kenne Kemene, T., Genva, M., Saive, M., Tonzibo, Z. F., et al., A new chemotype of Lantana rhodesiensis Moldenke essential oil from Côte d’Ivoire: Chemical composition and biological activities. Ind. Crops Prod., 141: 111766 (2019).

Khan, M., Mahmood, A. & Alkhathlan, H. Z., Characterization of leaves and flowers volatile constituents of Lantana camara growing in central region of Saudi Arabia. Arab. J. Chem., 9(6): 764–774 (2016).

Zandi-Sohani, N., Hojjati, M. & Carbonell-Barrachina, Ã. A., Bioactivity of Lantana camara L. essential oil against Callosobruchus maculatus (Fabricius). Chil. J. Agric. Res., 72(4): 502–506 (2012).

Shara, Y., Analisis Komponen Kimia Dan Uji Aktivitas Antibakteri Minyak Atsiri Bunga Tembelekan (Lantana camara L). Universitas Sumatera Utara, (2014).

Can BaÅŸer, K. H. & Buchbauer, G., Handbook of Essential Oils. Handbook of Essential Oils: Science, Technology, and Applications, Second Edition, CRC Press, (2015). doi:10.1201/b19393

González-Burgos, E., Carretero, M. E. & Gómez-Serranillos, M. P., Sideritis spp.: Uses, chemical composition and pharmacological activities—A review. J. Ethnopharmacol., 135(2): 209–225 (2011).

Yildirim, A., Cakir, A., Mavi, A., Yalcin, M., Fauler, G. & Taskesenligil, Y., The variation of antioxidant activities and chemical composition of essential oils of Teucrium orientale L. var. orientale during harvesting stages. Flavour Fragr. J., 19(5): 367–372 (2004).

Sharifi-Rad, J., Sureda, A., Tenore, G., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., et al., Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems. Molecules, 22(1): 70 (2017).

Dorman, H. J. D. & Deans, S. G., Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol., 88(2): 308–316 (2000).

Bakkali, F., Averbeck, S., Averbeck, D. & Idaomar, M., Biological effects of essential oils – A review. Food Chem. Toxicol., 46(2): 446–475 (2008).

Raineri, M., Histochemical localization of chitin in larvae of Artemia salina Leach (Phyllopoda). Bolletino di Zool., 48(2): 139–141 (1981).

R. Hamidi, M., Jovanova, B. & Kadifkova Panovska, T., Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Maced. Pharm. Bull., 60(01): 9–18 (2014).

Wiji Prasetyaningrum, P., Bahtiar, A. & Hayun, H., Synthesis and Cytotoxicity Evaluation of Novel Asymmetrical Mono-Carbonyl Analogs of Curcumin (AMACs) against Vero, HeLa, and MCF7 Cell Lines. Sci. Pharm., 86(2): 25 (2018).

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Published

2021-04-03

How to Cite

Suryati, S., Aziz, E. D., Efdi, M., Wahyuni, F. S., & Hefni, D. (2021). Analysis of The Essential Oil from Lantana camara Leaves and Its Cytotoxic Potential Against T-47D Breast Cancer Cells. Jurnal Riset Kimia, 12(1), 1–9. https://doi.org/10.25077/jrk.v12i1.364

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