Potensi Antimikroba dan Toksisitas Minyak Atsiri yang Diisolasi dari Biji Jintan (Carum carvi L.)

Authors

  • Suryati Suryati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia
  • Emil Salim Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia
  • Gustia Elizar Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia

DOI:

https://doi.org/10.25077/jrk.v13i2.551

Keywords:

Carum carvi L., essential oil, antimicrobial, cytotoxic

Abstract

Caraway (Carum carvi L.) is one of the oldest medicinal plants found in various countries such as West Asia, North Africa, Pakistan, India, North America, Northern Europe, Iran, Russia, and Indonesia. This plant has been use for traditional medicines to treat digestive disorders, flatulence, colic in infants, headaches, coughs, hypertension, eczema, pneumonia, diabetes, scabies, mouthwash and as an antiseptic. This plant is reported to contain around 1-9% essential oil in the seeds with various components and potential as bioactive compounds. In this study, essential oils were isolated from caraway seeds and determined their chemical content as well as their antimicrobial and toxicity. Isolation of essential oils was carried out by hydrodistillation and analysis of the chemical components of the isolated essential oils was carried out using the Gas Chromatography-Mass Spectrometry (GC-MS) method. The results of the GC-MS analysis showed the presence of 35 compounds with four main compounds, namely carvone (62.54%), limonene (23.39%), and trans-dihydrocarvone (8.20%). The antimicrobial activity test was carried out using the disc diffusion method, which showed strong activity against Staphylococcus aureus at a concentration of  ≥75%, and against the fungus Candida albicans. The results of the toxicity test using the Brine Shrimp Lethality Test (BSLT) method showed that caraway seed essential oil was low toxic against Artemia salina Leach shrimp larvae with an LC50 value 568.3292 mg/L.

Author Biography

Suryati Suryati, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas

Scopus Profile:

https://www.scopus.com/authid/detail.uri?authorId=57205311075

References

Donsì, F. & Ferrari, G., Essential oil nanoemulsions as antimicrobial agents in food. J. Biotechnol., 233: 106–120 (2016).

Okla, M. K., Alamri, S. A., Salem, M. Z. M., Ali, H. M., Behiry, S. I., Nasser, R. A., Alaraidh, I. A., et al., Yield, phytochemical constituents, and antibacterial activity of essential oils from the leaves/twigs, branches, branch wood, and branch bark of sour orange (Citrus aurantium L.). Processes, 7(6): (2019).

Efruan, G. K., Martosupono, M. & Rondonuwu S, F., Bioaktifitas Senyawa 1 , 8-Sineol pada Minyak Atsiri. Semin. Nas. Pendidik. dan Saintek 2016, 2016: 171–181 (2016).

Rasooli, I. & Allameh, A., in Essential Oils in Food Preservation, Flavor and Safety, (ed. Preedy, V. R.) (December): 287–293 (2016). doi:10.1016/B978-0-12-416641-7.00032-8

Goyal, M., Gupta, V. K., Singh, N. & Mrinal., Carum Carvi- An Updated Review. Indian J. Pharm. Biol. Res., 6(04): 14–24 (2018).

Sachan, A. K., Das, D. R. & Kumar, M., Carum carvi-An important medicinal plant. J. Chem. Pharm. Res., 8(3): 529–533 (2016).

Johri, R. K., Cuminum cyminum and Carum carvi: An update. Pharmacogn. Rev., 5(9): 63–72 (2011).

Khalil, N., Ashour, M., Fikry, S., Singab, A. N. & Salama, O., Chemical composition and antimicrobial activity of the essential oils of selected Apiaceous fruits. Futur. J. Pharm. Sci., 4(1): 88–92 (2018).

Lasram, S., Zemni, H., Hamdi, Z., Chenenaoui, S., Houissa, H., Tounsi, M. S. & Ghorbel, A., Antifungal and antiaflatoxinogenic activities of Carum carvi L., Coriandrum sativum L. seed essential oils and their major terpene component against Aspergillus flavus. Ind. Crops Prod., 134(March): 11–18 (2019).

Nasiri, S., Shams-ghahfarokhi, M. & Razzaghi-abyaneh, M., Inhibitory effect of Carum carvi essential oil on the growth of Candida albicans. Sci. J. Microbiol., 3(July): 74–77 (2014).

Begum, J., Bhuiyan, M. N. I., Chowdhury, J. U., Hoque, M. N. & Anwar, M. N., Antimicrobial Activity of Essential Oil from Seeds of Carum carvi and Its Composition. Bangladesh J. Microbiol., 25(2): 85–89 (2008).

Hajlaoui, H., Arraouadi, S., Noumi, E., Aouadi, K., Adnan, M., Khan, M. A., Kadri, A., et al., Antimicrobial, Antioxidant, Anti-Acetylcholinesterase, Antidiabetic, and Pharmacokinetic Properties of Carum carvi L. and Coriandrum sativum L. Essential Oils Alone and in Combination. Molecules, 2–18 (2021).

Aziz, E. D., Isolasi Triterpenoid dan Minyak Atsiri dari Daun Tumbuhan Lantana camara Linn serta Aktivitas Sitotoksiknya. Universitas Andalas, (2020).

Suryati., Aziz, E. D., Efdi, M., Wahyuni, F. S. & Hefni, D., Analysis of The Essential Oil from Lantana camara Leaves and Its Cytotoxic Potential Against T-47D Breast Cancer Cells. J. Risat Kim., 12: 1–9 (2021).

Ibrahim, S., Suryati. & Aziz, E. D., Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas ( Gluta renghas L ). J. Risat Kim., 11(1): 52–60 (2020).

Suryati., Bustanul, A. & Adriani, W., Penentuan Kandungan Fenolik Total, Uji Aktivitas Aantioksidan, Aktivitas Antimikroba, dan Sitotoksik dari Fraksi Metanol Daun Miana (Plectranthus scutellarioides (L.) R. Br). J. Kim. Unand, 7(2): 9–15 (2018).

Rahmi, M. & Felicia, A. A., Antifungal activity of onion ( Allium cepa L .) essential oil on Candida albicans. Ilmu Gizi Indones., 03(01): 59–64 (2019).

Laribi, B., Kouki, K., Bettaieb, T., Mougou, A. & Marzouk, B., Essential oils and fatty acids composition of Tunisian , German and Egyptian caraway ( Carum carvi L .) seed ecotypes : A comparative study. Ind. Crop. Prod., 41: 312–318 (2013).

Gajić, I., Stanojević, L., Dinić1, A., Stanojević, J., Nikolić, L., Nikolić, V. & Savić, V., The chemical composition of the essential oil and volatile compounds from caraway fruit ( Carum carvi L .) extracted by headspace-solid phase microextraction and the antioxidant activity. Adv. Technol., 9(July): 37–43 (2020).

Al-Rubaye, A. F., Kadhim, M. J. & Hameed, I. H., Phytochemical Profiles of Methanolic Seeds Extract of Cuminum cyminum using GC-MS Technique. Int. J. Curr. Pharm. Rev. Res., 8(02): 114–124 (2017).

Ahmed, A. F., Shi, M., Liu, C. & Kang, W., Comparative analysis of antioxidant activities of essential oils and extracts of fennel ( Foeniculum vulgare Mill .) seeds from Egypt and China. Food Sci. Hum. Wellness, 8(1): 67–72 (2019).

Pelczar, M. & Chan, E. C. S., Dasar-Dasar Mikrobiologi 2. UI Press, (2009).

Sudewi, S. & Lolo, W. A., Kombinasi Ekstrak Buah Mengkudu (Morinda Citrifolia L.) dan Daun Sirsak (Annona Muricata L.) dalam Menghambat Bakteri Escherichia Coli dan Staphylococcus Aureus (Combination of Noni Fruit Extract (Morinda Citrifolia L.) and Soursop Leaves (Annona Muricata. Kartika J. Ilm. Farm., 4(2): 36–42 (2016).

Morales, G., Sierra, P., Mancilla, A., Paredes, A., Loyola, L. A., Gallardo, O. & Borquez, J., Secondary metabolites from four medicinal plants from northern Chile: Antimicrobial activity and biotoxicity against Artemia salina. J. Chil. Chem. Soc., 48(2): 13–18 (2003).

Shahbazi, Y., Chemical Composition and In Vitro Antibacterial Activity of Mentha spicata Essential Oil against Common Food-Borne Pathogenic Bacteria. J. Pathog., 1–5 (2015). doi:10.1155/2015/916305

Diaz, K., Werner, E., Besoain, X., Flores, S., Donoso, V., Said, B., Nelson, C., et al., In Vitro Antifungal Activity and Toxicity of Dihydrocarvone-Hybrid Derivatives against Monilinia fructicola. antibiotics, 10(818): 11 (2021).

Al-Behadili, W. A. A., Faaz, R. A. & Tarmooz, A. A., Antifungal activity of Carum carvi L . extraction against Candidia albican and Aspergillus niger. Plant Arch., 19(November): 1799–1805 (2019).

R. Hamidi, M., Jovanova, B. & Kadifkova Panovska, T., Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Maced. Pharm. Bull., 60(01): 9–18 (2014).

Meyer, B. N.; Ferrigni, N. R.; Putnam, J. E.; Jacobsen, L. B.; Nichols, D. E. . & McLaughlin, L., Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. J. Med. Plant Reseach, 45: 31–32 (1982).

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Published

2022-09-30

How to Cite

Suryati, S., Emil Salim, & Gustia Elizar. (2022). Potensi Antimikroba dan Toksisitas Minyak Atsiri yang Diisolasi dari Biji Jintan (Carum carvi L.). Jurnal Riset Kimia, 13(2), 198–207. https://doi.org/10.25077/jrk.v13i2.551

Issue

Vol. 13 No. 2 (2022): September

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