AMPLIFIKASI GEN 16S-rRNA BAKTERI TERMOFILIK DARI SUMBER AIR PANAS, GUNUNG PANCAR BOGOR
DOI:
https://doi.org/10.25077/jrk.v3i1.97Abstract
Â
ABSTRACT
Â
Exploration of thermophilic bacteria that produce thermostable enzyme is most useful in application for enzyme base industrial. The aim of of this research is to isolate and amplificate the 16S-rRNA gene from thermophilic bacteria isolate at hotspring, Mount of Pancar, Bogor. The research steps consist of bacteria isolation, chromosomal DNA extraction, and amplification of 16S-rRNA gene. The water sample as source for bacteria was collected from four cauldrons. Temperature and pH for each cauldron are red cauldron 75-80°C, pH 7; black cauldron 55°C, pH 7; white cauldron 57°C, pH 7; and saline cauldron 25°C, pH 6, respectively. The bacteria were cultivated at Luria Bertani (LB) and Thermus media. The chromosomal DNA have been extracted. Gene amplification of 16 S-rRNA have been carried out by using universal primer (Bac F1 and Uni B1). The size of amplicon is ± 1.5kb.
Â
Keywords : thermophilic bacteria, chromosomal DNA extraction, amplification of 16S-rRNA gene
References
M. T. Madigan, B. L. Marrs, Extremophiles, Sci. Am., 82-87, (1997).
T. D. Brock, An overview of The Thermophiles. dalam Brock T D. Thermophiles: General, Molecular and Applied Microbiology, Jhon Wiley & Sons, New York, 1986.
R. I. Amann, W. Ludwig, K. H. Schleifer, Identification of uncultured bacteria: a challenging task for molecular taxonomist, ASM News, 60: 360-365, (1994).
T. Desiliyarni, Analisis keragaman genetik bakteri termofilik dari Kawah Candradimuka, Pegunungan Dieng dengan teknik PCR-RFLP Gen 16S-rRNA, [Tesis], Bogor: Program Pasca Sarjana, Institut Pertanian Bogor, 1999.
E. C. Bottger, Approaches for identification of microorganisms, ASM News, 62: 247-250, (1996).
R. G. Taylor, Polymerase Chain Reaction: Basic Principles and Automation, Oxford University Press, Oxford New York, 1992.
Indrajaya et al., Isolasi dan identifikasi mikroorganisme termofil isolat Kawah Wayang, Jurnal Mikrobiologi Indonesia, 8: 53-56, (2003).
M. W. W. Adams and R. M. Kelly, Enzymes from microorganisms in extreme environments, Chemical and Engineering News (reprints), 1995.
Downloads
Published
How to Cite
Issue
Section
Citation Check
License
Please find the rights and licenses in Jurnal Riset Kimia (J. Ris. Kim). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.
1. License
The use the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License.Â
2. Author(s)' Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
Under the Creative Commons license, the journal permits users to copy, distribute, and display the material for any purpose. Users will also need to attribute authors and J. Ris. Kim on distributing works in the journal and other media of publications.
4. Rights of Authors
Authors retain all their rights to the published works, such as (but not limited to) the following rights;
- Copyright and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in own future works, including lectures and books,
- The right to reproduce the article for own purposes,
- The right to self-archive the article,
- The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
5. Co-Authorship
If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. J. Ris. Kim will not be held liable for anything that may arise due to the author(s) internal dispute. J. Ris. Kim will only communicate with the corresponding author.