VALIDASI METODA FRAP MODIFIKASI PADA PENENTUAN KANDUNGAN ANTIOKSIDAN TOTAL DALAM SAMPEL MANGGA DAN RAMBUTAN

Authors

  • Yefrida - Laboratorium Kimia Analisa Terapan, Jurusan Kimia FMIPA, Universitas Andalas Jurusan Kimia FMIPA Unand, Kampus Limau Manis, 25163
  • Nor Ashikin Laboratorium Kimia Lingkungan, Jurusan Kimia FMIPA, Universitas Andalas Jurusan Kimia FMIPA Unand, Kampus Limau Manis, 25163
  • Nor Ashikin Laboratorium Kimia Analisa Terapan, Jurusan Kimia FMIPA, Universitas Andalas Jurusan Kimia FMIPA Unand, Kampus Limau Manis, 25163
  • Refilda - Laboratorium Kimia Lingkungan, Jurusan Kimia FMIPA, Universitas Andalas Jurusan Kimia FMIPA Unand, Kampus Limau Manis, 25163
  • Refilda - Laboratorium Kimia Analisa Terapan, Jurusan Kimia FMIPA, Universitas Andalas Jurusan Kimia FMIPA Unand, Kampus Limau Manis, 25163

DOI:

https://doi.org/10.25077/jrk.v8i2.236

Keywords:

antioxidants, modified FRAP method, validation method

Abstract

Validation of modified FRAP method to determine total antioxidant content in mango and rambutan sample have been done. Total antioxidan content in sampel is presented as Gallic Acid Equivalent (GAE). Modified FRAP method using FeCl3asoxidizing agent and ortho-phenantroline as complexan, which produce complex compounds of [Fe (C12H8N2)3]+2 sorrel coloured. Validation parameters are obtained in this research are r = 0.999, R2 = 0.998, LoD 3.13 x 10-6 M and LoQ 1.04 x 10-5 M. While RSD and recovery in the sample mango and rambutan were 1.15%, 1.34% and 96.31%, 97.72%, respectively. Based on the value of the validation parameters, it can be concluded that this method can be used for determination of total antioxidant content in the mango and rambutan samples.

References

K. Mahattanatawee, J.A. Manthey, G. Luzio, S.T. Talcott, K. Goodner, E. A. Baldwin, Total antioxidant activity and fiber content of select Florida-grown tropical fruits, J. Agr. & Food Chem., 54(19), 7355–7363 (2006)

S.M.R. Ribeiro, J.H. Queiroz , M.E.L. Ribeiro de Queiroz , F.M. Campos , H.M.P. Sant'ana, Antioxidant in mango (Mangifera indica L.) pulp. Plant Foods Hum Nutr. 62 (1) : 13-17 (2007)

B. Tepe, S. Degerli, S. Arslan, E. Malatyali, C. Sarikurkcu, Determination of chemical profile, antioxidant, DNA damage protection and antiamoebic activities of Teucrium polium and Stachys iberica, Fitoterapia, 82 : 237–246 (2011)

A. Garcia, M.G. Alriols, G. Spigno, J. Labidi, Lignin as natural radical scavenger. Effect of the obtaining and purification processes on the antioxidant behaviour of lignin, Biochem. Eng. J., 67 : 173– 185 (2012)

S. Flis, Z. Jastrzebski, J. Namiesnik, P. Arancibia-Avilac, F. Toledo, H. Leontowicz, M. Leontowicz, M. Suhaj, S. Trakhtenberg, S. Gorinstein, Evaluation of inhibition of cancer cell proliferation in vitro with different berries and correlation with their antioxidant levels by advanced analytical methods, J. Pharm. Biomed. Anal. 62 : 68– 78 (2012)

G.F. Deng, X.R. Xu, Y.J. Guo, E.Q. Xiaa, S. Lia, S. Wu, F. Chen, W.H. Linga, H. B. Li, Determination of antioxidant property and their lipophilic and hydrophilic phenolic contents in cereal grains, J. Func. Food., 4 : 906 –914 (2012)

P. Biparva, M. Ehsani, M.R. Hadjmohammadi Dispersive liquid–liquid microextraction using extraction solvents lighter than water combined with high performance liquid chromatography for determination of synthetic antioxidants in fruit juice samples, J. Food Compos. Anal., 27 : 87–94 (2012)

M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernández-López, E. Sendra, E. Sayas-Barberá, J.A. Pérez-Ãlvarez, Antioxidant properties of pomegranate (Punica granatum L.) bagasses obtained as co-product in the juice extraction, Food Res. Int., 44 : 1217–1223 (2011)

R. Martínez, P. Torres, M.A. Meneses, J.G. Figueroa, J.A. Pérez-Ãlvarez , M. Viuda-Martos Chemical, technological and in vitro antioxidant properties of cocoa (Theobroma cacao L.) co-products, Food Res. Int. 49 : 39-45 (2012)

E. Bursal, E. Köksal, İ. Gülçin, G. Bilsel, A.C. Gören, Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC–MS/MS, F. Res. Int., 51 : 66–74 (2013)

V. Kraujalyte, P.R. Venskutonis, A. Pukalskas, L. Cesoniene, R. Daubaras Antioxidant properties, phenolic composition and potentiometric sensor array evaluation of commercial and new blueberry (Vaccinium corymbosum) and bog blueberry (Vaccinium uliginosum) genotypes, Food Chem.188 : 583–590 (2015)

M. R. Loizzo, A. Pugliese, M. Bonesi, F. Menichini, R. Tundis, Evaluation of chemical profile and antioxidant activity of twenty cultivars from Capsicum annuum, Capsicum baccatum, Capsicum chacoense and Capsicum chinense: A comparison between fresh and processed peppers, LWT - Food Sci. Techn., 64 : 623-631 (2015)

T.D.T. Do, D. Cozzolino, B. Muhlhausler, A. Box, A.J. Able, Antioxidant capacity and vitamin E in barley: Effect of genotype and storage, Food Chem.,187 : 65–74 (2015)

A. SzydÅ‚owska-Czerniak, A. Åaszewska, Effect of refining process on antioxidant capacity, total phenolics and prooxidants contents in rapeseed oils, LWT - Food Sci. Tech., 64 : 853-859 (2015)

A. Szydłowska-Czerniak, A. Tułodziecka, Comparison of a silver nanoparticle-based method and the modified spectrophotometric methods for assessing antioxidant capacity of rapeseed varieties, Food Chem., 141:1865–1871 (2013).

T. Alfonso de Araujo, A.M.J.Barbosa, L.H. Viana, V.S. Ferreira Electroanalytical determination of TBHQ , a synthetic antioxidant, in soybean biodiesel samples, Fuel 90 : 707–712 (2011).

S.S. Marques, I.V. Tóth, L.M. Magalhães, S. Reis, M.A. Segundo, High-sensitivity programmable flow method for assessment of total antioxidant capacity in biological samples, Microchem. J., 124: 261–266 (2016)

P. Biparva, M. Ehsani, M.R. Hadjmohammadi, Dispersive liquid–liquid microextraction using extraction solvents lighter than water combined with high performance liquid chromatography for determination of synthetic antioxidants in fruit juice samples, J. Food Compos. Anal. 27 : 87–94 (2012).

A. Aktas_ Karacelik, M. Kucuk, Z. Iskefiyeli, S. Aydemir, S. De Smet, B. Miserez , P. Sandra, Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods, Food Chem., 175 : 106–114 (2015)

M. Pisoschi1, G.P. Negulescu, Methods for Total Antioxidant Activity Determination: A Review, Biochem & Anal Biochem, 1:1, 1-10 (2011) .

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Published

2015-03-15

How to Cite

-, Y., Ashikin, N., Ashikin, N., -, R., & -, R. (2015). VALIDASI METODA FRAP MODIFIKASI PADA PENENTUAN KANDUNGAN ANTIOKSIDAN TOTAL DALAM SAMPEL MANGGA DAN RAMBUTAN. Jurnal Riset Kimia, 8(2), 170. https://doi.org/10.25077/jrk.v8i2.236

Issue

Vol. 8 No. 2 (2015): March

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Articles

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