Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang)

Adlis Santoni, Mai Efdi, Akmel Suhada

Abstract

Hornstedtia scyphifera var. fusiformis Holttum is endemic plant of Zingiberaceae family that distributed in Sumatera. Hornstedtia scyphifera var. fusiformis Holttum contained some secondary metabolite such as phenolic, saponin, triterpenoid and alkaloid. The biological activity of methanol, ethyl acetate, and n-hexane extracts from Hornstedtia scyphifera var. fusiformis Holttum suspended roots was tested for antibacterial and antioxydant activity. Antioxidant activity was analized by DPPH method. The antibacterial activity  was tested by used disk difussion method againts Staphylococcus aureus bacteria (gram-positive bacteria) and Escherichia coli (gram-negative bacteria). Almost all of Hornstedtia scyphifera var. fusiformis Holttum suspended roots extract were given antibacterial activity, nonetheless the biggest inhibition zone of Escherichia coli that was inhibited by n-hexane and ethyl acetate extracts which have inhibtion zone 10 mm at  concentration 500 mg/L and also againts Staphylococcus aureus, the biggest inhibition by ethyl acetate and n-hexane extracts which have inhibition zone 10.30 mm at concentration 500 mg/L. Among all extracts tested, methanol extract of the possessed moderate free radical scavenging activity with IC50 = 51.89 mg/L.

Keywords

Hornstedtia scyphifera var. fusiformis Holttum; antibacterial; antioxidant

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References

Karumaran, S., Nethaji, S. & Rajakumar, R., Antimicrobial and antioxidant activity of leaf extracts of Aegle marmelos. 7(3): 205–208 (2016).

Julie, J. & Ernest, T. J., Evaluation of Antioxidant Potential of Rhizome Extracts of Two Species of Alpinia Roxb. (Zingiberacea). (March): (2019).

Kamazeri, T. S. A. T., Samah, O. A., Taher, M., Susanti, D. & Qaralleh, H., Antimicrobial activity and essential oils of Curcuma aeruginosa, Curcuma mangga, and Zingiber cassumunar from Malaysia. Asian Pac. J. Trop. Med., 5(3): 202–209 (2012).

Natta, L., Orapin, K., Krittika, N. & Pantip, B., Essential oil from five Zingiberaceae for anti food-borne bacteria. Int. Food Res. J., 15(3): 337–346 (2008).

Holttum, R. E., The zingiberaceae of the Malay Peninsula. Gard. Bull. Singapore, 13(1): 1–249 (1950).

Hashim, S. E., Sirat, H. M. & Yen, K. H., Chemical compositions and antimicrobial activity of the essential oils of Hornstedtia havilandii (Zingiberaceae). Nat. Prod. Commun., 9(1): 119–120 (2014).

Jani, N. A., Ibrahim, N., Hashim, S. E. & Sirat, H. M., Antimicrobial and Antioxidant Activities of Hornstedtia leonurus Retz. Extracts. J. Sci. Technol., 7(2): (2015).

Lestari, T., Nurainas, N. & Syamsuardi, S., Studi Morfometrik Hornstedtia leonurus (J. Koenig) Retz.(Zingiberaceae) dan Kerabat Dekatnya dalam Tribe Alpiniae di Sumatera Barat. J. Biol. Univ. Andalas, 4(3): (2015).

Tagashira, M. & Ohtake, Y., A new antioxidative 1, 3-benzodioxole from Melissa officinalis. Planta Med., 64(06): 555–558 (1998).

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