EKSTRAKSI DAN IDENTIFIKASI SENYAWA ANTOSIANIN DARI JANTUNG PISANG RAJA (Musa x paradisica L.) SERTA UJI AKTIVITAS ANTIOKSIDANNYA

Jessica Alvionita, Djaswir Darwis, Djaswir Darwis, Mai Efdi

Abstract

Anthocyanin coumpound have been successfully extracted from plantain bud (Musa x paradisiaca L) which can used as natural colorant and antioxidant. Coumpound anthocyanin exracted using maceration method with solvent ethanol that has been acidified with acetic acid and citric acid until pH 1,5. The extract anthocyanin is identified by UV-Vis spectrophotometer (260-800 nm) and retrieved two peaks that is 277 nm (UV) and 533 nm (Visible). Further treatment is carried out with KCKT-DAD at a wavelength 516 nm. Anthocyanin treatment given temperature and pH. Total anthocyanin monomeric obtained for ethanol + acetic acid was 30,22 mg/L; ethanol + citric acid was 18,20 mg/L. The warming influence of the anthocyanin compound lead to degradation of color is highest for ethanol + acetic citric on temperature 100oC with percentage degradation of 61,97 %. The antioxidant activity of the extract was measured by 1,1- difenil-2-pikrilhidrazil (DPPH) the result showed that the anthocyanin extracts from Plantain bud has antioxidant activity ethanol + acetic citric acid extract is very active as an antioxidant with IC50  values of 3,74 mg/L.

Keywords

Musa x paradisiaca L., Anthocyanin, Degradation of Color

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References

Winarno, G., 2004. Kimia Pangan dan Gizi. PT Gramedia, Jakarta.

Yoga, Matius Kristiarso, Wibowo Catur. Antosianin Jantung Pisang Kepok (Musa x Paradisiaca) Sebagai Pewarna Agar-Agar (Kajian Berdasarkan Stabilitas Terhadap Cahaya Dan Organoleptik). Salatiga.

Barnes, Jeremi S., 2010. Analytical Characterization of Anthocyanins from Natural Product by Reverse-Phase Liquid Chromatography-Photoidiode Addar-Electrospray Ionization-Ion of Flight Mass Spewctrometry. Master of Science in Chemistry. The University of Texas at Arlington.

Lestario, Danu dan Kris., 2009. Kandungan Antosianin dan Antosianidin dari Jantung Pisang Klutuk ( Musa brachycarpa Back) dan Pisang Ambon (Musa acuminata Colla). Jurnal Teknologi dan Industry Pangan. Salatiga. Volume XX, No. 2, Hal 143 – 148.

Armour publishing Pte Ltd., 2009., Tress of our garden city. National parks Board. Singapore. Edition 2nd. Hal 184-185.

Huang, Wen Jian, Shao-Ling Zhang., Gai-Hua Qin,Le Wenqua, Jun Wu., 2012. Isolation and determination of Major Anthocyanin Pigment in The Pericarp of P. Communis L. Cv. ‘Red Du Comices’ and Their Associatio with Antioxidant Activity. African Journal of Agricultural Research, Vol. 7 (26),3772- 3780.

Supiyanti, Wiwin, E. D., Dwi Wulansari,dan Lia Kusmita.,2010. Uji Aktivitas Antioksidan dan Penentuan Kandungan Antosianin Total Kulit Buah Manggis (Garcinia Mangostana L). Majalah Obat Tradisional, 15(2), 64 – 70.

Anonim. 2012. Stabilitas Antosianin Buah Duwet (Syzygium cumini) Dalam Minuman Model. Jurnal IPB, Bogor.

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