Penetapan Kadar Fenolik dan Flavonoid Hasil Ultrasonic Assisted Extraction Daun Binahong (Anredera cordifolia [Ten] Steenis)

Authors

  • Landyyun Rahmawan Sjahid Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, Indonesia, Indonesia
  • Amelia Aqshari Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, Indonesia, Indonesia
  • Sediarso Sediarso Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. Dr. Hamka, Jakarta, Indonesia, Indonesia

DOI:

https://doi.org/10.25077/jrk.v11i1.348

Keywords:

Ultrasonic assisted extraction, Anredera cordifolia [Ten] Steenis, phenolic, flavonoid

Abstract

Anredera cordifolia [Ten] Steenis has been proven to have pharmacological activity that is thought to be influenced by the presence of phenolic compounds and flavonoids. However, both compounds are found in small concentrations through conventional extraction (maceration, decoction, soxhlet, and reflux). This study aims to prove that the ultrasonic assisted extraction method can obtain higher concentrations of phenolic and flavonoid compounds. Dried binahong leaves were extracted using ultrasonic assisted extraction with 70% ethanol. Phenolic levels were determined using Folin-Ciocalteu, while flavonoid levels were determined using the colorimetric aluminum method. The results showed binahong leaves ethanolic extract had phenolic levels of 10.16 % GAE and flavonoid levels of 10.58 % QE. These results are much higher than the results obtained using conventional extraction so that ultrasonic assisted extraction can be recommended to maximize the extraction of phenolic and flavonoid levels from the leaves of Binahong (Anredera cordifolia [Ten] Steenis). 

References

Wijayanti, K. & Esti, R. H. S., Effectiveness of binahong decoction water (Anredera cordifolia (Ten.) Steenis) for perineal wound healing at home delivery aesya grabag Magelang, Indonesia. Int. J. Res. Med. Sci., 5(5): 1970–1975 (2017).

Sutrisno, E., Sukandar, E., Fidrianny, I. & Adnyana, I. K., Wound healing in vivo and in vitro study of binahong leaves (Anredera cordifolia (ten.) Steenis) and pegagan (Centella asiatica (L.) Urban) ethanolic extract. Pharmacol. OnLine, 1: 111–116 (2018).

Wijayanti, D., Setiatin, E. T. & Kurnianto, E., Leucocyte profile and offspring production of Guinea pig (Cavia cobaya) given Anredera cordifolia leaf extract. J. Indones. Trop. Anim. Agric., 43(1): 19–25 (2018).

Yuziani., Harahap, U. & Karsono., Evaluation of analgesic activities of ethanolic extract of Anredera cordifolia (Ten.) v. Steenis leaf. Int. J. PharmTech Res., 6(5): 1608–1610 (2014).

Sutrisno, E., Adnyana I, K., Yulinah Sukandar, E., Fidrianny, I. & Aligita, W., Anti-inflammatory study of Anredera cordifolia leaves and Centella asiatica herbs and its combinations using human red blood cell-membrane stabilization method. Asian J. Pharm. Clin. Res., 9(5): 78–80 (2016).

Fidrianny, I., Wirasutisna, K. R. & Amanda, P., Senyawa antioksidan dari ekstrak etil asetat daun binahong (Anredera cordifolia (Ten.) Steenis) dari Babakan Ciparay, Bandung Selatan, Indonesia. Acta Pharm. Indones., 38(1): 26–30 (2013).

Susanti, H., Total phenolic content and antioxidant activities of binahong (Anredera cordifolia.). J. Kedokt. dan Kesehat. Indones. (Indonesian J. Med. Heal., 10(2): 171–175 (2019).

Estowo, R., Zulfian. & Tjiptaningrum, A., The effect of binahong leaves extraction (Anredera Cordifolia (Ten.) Steenis) in 70 % ethanol toward ALT activities of male rat (Rattus norvegicus) of Sprague dawley which has induced by ethanol 50 %. Majority, 3(6): 108–117 (2014).

Wibhisono, H., Busman, H. & Susantiningsih, T., Efek protektif ekstrak etanol daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap gambaran histopatologi lambung tikus putih galur Sprague dawley yang diinduksi etanol. Majority, 3(6): 170–178 (2014).

Sukandar, E. Y., Fidrianny, I. & Adiwibowo, L. F., Efficacy of ethanol extract of Anredera cordifolia (Ten.) Steenis leaves on improving kidney failure in rats. Int. J. Pharmacol., 7(8): 850–855 (2011).

Leliqia, N. P. E., Sukandar, E. Y. & Fidrianny, I., Antibacterial activities of Anredera cordifolia (Ten.) v. Steenis leaves extracts and fractions. Asian J. Pharm. Clin. Res., 10(12): 10–13 (2017).

Basyuni, M., Ginting, P. Y. A. B. & Lesmana, I., Phytochemical analysis of binahong (Anredera cordifolia) leaves extract to inhibit in vitro growth of Aeromonas hydrophila. in Proceedings of the 3rd International Symposium on Applied Chemistry, 1904: 020072–1–020072–5 AIP Publishing, (2017).

Elyani, H. & Risandiansyah, R., Antibacterial potential of four herbal plants (Syzygium cumini, Piper ornatum, Anredera cordifolia, and Alpinia galangan) against Staphylococcus aureus and Escherichia coli. JIMR - J. Islam. Med. Res., 1(2): 26–33 (2017).

Djamil, R., Winarti, W., Zaidan, S. & Abdillah, S., Antidiabetic activity of flavonoid from binahong leaves (Anredera cordifolia) extract in alloxan induced mice. J. Pharmacogn. Nat. Prod., 03(02): 139 (2017).

Astuti, S. M., Sakinah, A. M. M. & Risch, A., The triterpenoid saponin from binahong [Anredera cordifolia (Ten) Steenis] to potential using as antidiabetic activity in animal laboratory. in International Conference on Drug Development of Natural Resources, 331–344 (2012).

Lestari, D., Sukandar, E. Y. & Fidrianny, I., Anredera cordifolia leaves extract as antihyperlipidemia and endothelial fat content reducer in male Wistar rat. Int. J. Pharm. Clin. Res., 7(6): 435–439 (2015).

Garmana, A. N., Sukandar, E. Y. & Fidrianny, I., Antihypertension study of Anredera cordifolia (Ten.) v. Steenis extract and its fractions in rat through dexamethasone induction and nitric oxide release. Asian J. Pharm. Clin. Res., 11(1): 278–282 (2018).

Pitaloka, D. A. E. & Sukandar, E. Y., Synergistic study on n-hexane extract of Anredera cordifolia (Ten.) v Steenis (binahong) leaves combined with antituberculosis drugs against drug-sensitive and drug-resistant of Mycobacterium tuberculosis. J. Appl. Pharm. Sci., 8(5): 134–138 (2018).

Virginia, D. M., Saputra, R. P. & Setiawati, A., Cytotoxic activity of Anredera cordifolia leaf extract on Hela cells. Biotropia (Bogor)., 26(1): 33–40 (2019).

Mulia, K., Muhammad, F. & Krisanti, E., Extraction of vitexin from binahong (Anredera cordifolia (Ten.) Steenis) leaves using betaine - 1,4 butanediol natural deep eutectic solvent (NADES). in International Conference on Chemistry, Chemical Process and Engineering (IC3PE), 1823: 020018–1–020018–4 (2017).

Dwitiyanti., Harahap, Y., Elya, B. & Bahtiar, A., Impact of solvent on the characteristics of standardized binahong leaf (Anredera cordifolia (Ten.) Steenis). Pharmacogn. J., 11(6): 1463–1470 (2019).

Bari, I. N., Kato-Noguchi, H., Iwasaki, A. & Suenaga, K., Allelopathic potency and an active substance from Anredera cordifolia (Tenore) Steenis. Plants, 8(134): 1–9 (2019).

Manik, Y., Fachriyah, E. & Kusrini, D., Isolasi, identifikasi dan uji sitotoksik senyawa steroid dari daun binahong (Anredera cordifilia (Ten.) Stennis). J. Kim. Sains dan Apl., 16(1): 23–26 (2013).

Souza, L. F., Barros, I. B. I. de., Mancini, E., Martino, L. De., Scandolera, E. & Feo, V. De., Chemical composition and biological activities of the essential oil from Anredera cordifolia grown in Brazil. Nat. Prod. Commun., 9(7): 1003–1006 (2014).

Astuti, S. M., Sakinah A.M, M. S., Andayani B.M, R. & Risch, A., Determination of saponin compound from Anredera cordifolia (Ten) Steenis plant (binahong) to potential treatment for several diseases. J. Agric. Sci., 3(4): 224–232 (2011).

Veronita, F., Wijayati, N. & Mursiti, S., Isolasi dan uji aktivitas antibakteri daun binahong serta aplikasinya sebagai hand sanitizer. Indones. J. Chem. Sci., 6(2): 138–144 (2017).

Djamil, R., Wahyudi, P., Wahono, S. & Hanafi, M., Antioxidant activity of flavonoid from Anredera cordifolia (Ten) Steenis leaves. Int. Res. J. Pharm., 3(9): 241–243 (2012).

Djamil, R., Winarti, W., Syamsudin. & Rasna, M., Standardization and A-glucosidase inhibitory of extract from Anredera cordifolia leaves. in Proceedings of The 9th Joint Conference on Chemistry, 317–321 (2015).

Hasri., Anwar, M. & Karim, M., Analisis fenolik dan daya hambat daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap bakteri Eschericia coli dan Staphylococcus aureus. Indones. Chem. Appl. J., 1(1): 1–9 (2017).

Selawa, W., Runtuwene, M. R. J. & Citraningtyas, G., Kandungan flavonoid dan kapasitas antioksidan total ekstrak etanol daun binahong [Anredera cordifolia (Ten.) Steenis.]. Pharmacon J. Ilm. Farm. UNSRAT, 2(1): 18–22 (2013).

Susanty. & Yudhistirani, S. A., Pengaruh waktu ekstraksi daun binahong (Anredera cordifolia (Tenore) Steenis) terhadap kemampuan daya hambat bakteri Escherichia coli untuk pembuatan hand sanitizer. J. Konversi, 7(1): 1–10 (2018).

Nipornram, S., Tochampa, W., Rattanatraiwong, P. & Singanusong, R., Optimization of low power ultrasound-assisted extraction of phenolic compounds from mandarin (Citrus reticulata Blanco cv. Sainampueng) peel. Food Chem., 241: 338–345 (2018).

Pudziuvelyte, L., JakÅ¡tas, V., Ivanauskas, L., LaukeviÄienÄ—, A., Ibe, C. F. D., Kursvietiene, L. & Bernatoniene, J., Different extraction methods for phenolic and volatile compounds recovery from Elsholtzia ciliata fresh and dried herbal materials. Ind. Crops Prod., 120: 286–294 (2018).

Oniszczuk, A., Podgórski, R., Oniszczuk, T., Zukiewicz-Sobczak, W., Nowak, R. & Waksmundzka-Hajnos, M., Extraction methods for the determination of phenolic compounds from Equisetum arvense L. herb. Ind. Crops Prod., 61: 377–381 (2014).

Winata, E. W. & Yunianta., Ekstraksi antosianin buah murbei (Morus alba L.) metode ultrasonic bath (kajian waktu dan rasio bahan : pelarut). J. Pangan dan Agroindustri, 3(2): 773–783 (2015).

Departemen Kesehatan Republik Indonesia., Buku panduan teknologi ekstrak. Direktorat Jenderal Pengawasan Obat dan Makanan, (2000).

Pasaribu, G., Gusmailina., Komarayati, S., Zulnely. & Dahlian, E., Analisis senyawa kimia Dryobalanops aromatica. J. Penelit. Has. Hutan, 32(1): 21–26 (2014).

Departemen Kesehatan Republik Indonesia., Farmakope herbal indonesia. Direktorat Jenderal Pengawasan Obat dan Makanan, (2008).

Siddiqui, N., Rauf, A., Latif, A. & Mahmood, Z., Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gul-e-Zoofa (Nepeta bracteata Benth). J. Taibah Univ. Med. Sci., (2017). doi:10.1016/j.jtumed.2016.11.006

Chang, C.-C., Yang, M.-H., Wen, H.-M. & Chern, J.-C., Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 10(3): 178–182 (2002).

Kementrian Kesehatan RI., Farmakope herbal indonesia. Kementrian Kesehatan RI, (2011).

Singleton, V. L., Orthofer, R. & Lamuela-Raventós, R. M., Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol., 299: 152–178 (1999).

Schofield, P., Mbugua, D. M. & Pell, A. N., Analysis of condensed tannins : a review. Anim. Feed Sci. Technol., 91: 21–40 (2001).

Chen, L. Y., Cheng, C. W. & Liang, J. Y., Effect of esterification condensation on the Folin-Ciocalteu method for the quantitative measurement of total phenols. Food Chem., 170: 10–15 (2015).

Cornard, J. P. & Merlin, J. C., Complexes of aluminium (III) with isoquercitrin : spectroscopic characterization and quantum chemical calculations. Polyhedron, 21: 2801–2810 (2002).

Chemat, F., Rombaut, N., Sicaire, A. G., Meullemiestre, A., Fabiano-Tixier, A. S. & Abert-Vian, M., Ultrasound assisted extraction of food and natural products. mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34: 540–560 (2017).

Nikolovska-Čoleska, Ž., Dorevski, K., Klisarova, L. & Šuturkova-Milošević, L., Identification of phenolic constituents isolated from Macedonian propolis. Bull. Chem. Technol. Maced., 14(1): 13–17 (1995).

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Published

2020-03-30

How to Cite

Sjahid, L. R., Aqshari, A., & Sediarso, S. (2020). Penetapan Kadar Fenolik dan Flavonoid Hasil Ultrasonic Assisted Extraction Daun Binahong (Anredera cordifolia [Ten] Steenis). Jurnal Riset Kimia, 11(1), 16–23. https://doi.org/10.25077/jrk.v11i1.348

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