Pengaruh Penambahan Surfaktan pada Fermentasi Tandan Kosong Kelapa Sawit dengan Praperlakuan Uap Air Panas Bertekanan dengan Katalis Asam Maleat

Sita Heris Anita, Devi Nury Ashrianis, Widya Fatriasari

Abstract

Oil palm empty fruit bunch (OPEFB) has high potency to be converted into second generation bioethanol production. OPEFB pretreated liquid hot water (LHW) assisted maleic acid (MA) pretreatment was used as substrate in simultaneous saccharification fermentation (SSF). This pretreatment was conducted at 180 °C for 70 min by using 1.5% (w/v) MA acid concentration with material and liquid ratio of 1:14. Subsequently, the pretreated samples were fermented by addition of surfactant, polyethylene glycol (PEG) 4000 and amphiphilic lignin derivatives (A-LD) using Kluyveromyces marxianus InaCC Y119 yeast at temperature of 38 °C for 89 h. Before SSF, pretreated OPEFB was pre hydrolyzed by cellulase enzyme with enzyme loading 30 FPU/g at 50 °C for 3 h. Fermentation filtrate was taken at 0, 24, 48, 72, dan 89 h as much as 1.5 mL for reducing sugar and ethanol content determination. The surfactant addition can enhance reducing sugar and also ethanol content compared to control. The highest ethanol content of 1.251 g/L was found at fermentation of OPEFB pretreated MA with PEG 4000 surfactant at 89 h.

Keywords

Maleic acid; SSF; Lignocellulose; LHW; Biosurfactant

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References

Novia, Windarti, A, & Rosmawati, Pembuatan bioetanol dari jerami padi dengan metode ozonolisis – simultaneous saccharification and fermentation (SSF). JTK, 20(3): 38–48 (2014).

Sari, F.P, Sholihat, N.N., Anita, S.H, Fitria, & Hermiati, E., Peningkatan produksi gula pereduksi dari tandan kosong kelapa sawit dengan praperlakuan asam organik pada reaktor bertekanan. Reaktor, 16(4): 199–206 (2016).

Fatriasari, W., Raniya, R., Oktaviani, M., & Hermiati, E., The improvement of sugar and bioethanol production of oil palm empty fruit bunches (Elaeis guineensis Jacq) through microwave-assisted maleic acid pretreatment. Bioresour., 13(2): 4378–4403 (2018).

Erivianto, D., Abhi, P.B., & Notosudjono, D., Penggunaan limbah kelapa sawit untuk menghasilkan tenaga listrik pada existing boiler. Saintech., 26(2): 85–95 (2016).

Hermiati, E., Mangunwidjaja, D., Sunarti, T.C., Suparno, O., & Prasetya, B., Pemanfaatan biomassa lignoselulosa ampas tebu untuk produksi bioetanol. J. Litbang Pertan., 29(4): 121–130 (2010).

Yu, Q., Zhuang, X., Lv, S., He, M., Zhang, Y., Yuan, Z ., Qi, W., Wang, Q., Wang, W., & Tan, X., Liquid hot water pretreatment of sugarcane bagasse and its comparison with chemical pretreatment methods for the sugar recovery and structural changes. Bioresour.Technol., 129: 592–598 (2013).

Anita, S.H, Oktaviani, M., & Hermiati, E., Bioethanol production from pretreated oil palm empty fruit bunches of SHF and SSF system by using ethanologenic yeast. Jurnal Lignocellul. Technol., (2018).

Li, J, Li, S., Fan, C., & Yan, Z., The mechanism of poly (ethylene glycol) 4000 effect on enzymatic hydrolysis of lignocellulose. Coloids Surf. B., 89: 203–210 (2012).

Börjesson, J, Engqvist, M., Sipos, B., & Tjerneld, F., Effect of poly (ethylene glycol) on enzymatic hydrolysis and adsorption of cellulase enzymes to pretreated lignocellulose. Enzym. Microb. Technol., 41: 186–195 (2007).

Zhou, Y.H., Chen, F., Qi, X., Zhao, & Liu, D., Non-ionic surfactants do not consistently improve the enzymatic hydrolysis of pure cellulose. Bioresour. Technol., 18: 136–140 (2015).

Winarni, I, Komarayati, S., & Bardant, T. B., Pembuatan bioetanol secara enzimatis dari limbah batang sawit (Elaeis guineensis) dengan penambahan surfaktan. J. Penelit. Has. Hutan, 34(2): 127–135 (2016).

Cheng, N, Koda, K., Tamai, Y., Yamamoto, Y., Takasuka, T.E., & Uraki, Y., Optimization of simultaneous saccharification and fermentation conditions with amphipilic lignin derivatives for concentrated bioethanol production. Bioresour. Technol., 232: 126–132 (2017).

Dowe, N. & Mcmillan, J., SSF experimental protocols — lignocellulosic biomass hydrolysis and fermentation laboratory analytical procedure (LAP) NREL/TP-510-42630. (2008).

Miller, G. L., Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31(3): 426–428 (1959).

Hermiati, E., Rekayasa proses hidrolisis ampas tapioka menggunakan pemanasan gelombang mikro untuk produksi etanol. Institut Pertanian Bogor, (2012).

Liu, Y., Xu, J., Zhang, Y., He, M., Liang, C., Yuan, Z., & Xie, J., Improved ethanol production based on high solids fed-batch simultaneous saccharification and fermentation with alkali-pretreated sugarcane bagasse. Bioresour., 11(1): 2548–2556 (2016).

Hidayat, M. R., Teknologi pretreatment bahan lignoselulosa dalam proses produksi bioetanol. Biopropal Ind., 4(1): 33–48 (2013).

Fatriasari, W., Karimah, A., Falah, F., Anita, S. H., Effect of amphiphilic lignin derivatives (A-LD) surfactant addition on the fermentation process of sorghum bagasse kraft pulp for bioethanol production. in The IOP Conference Series: Earth and Environmental Science (EES), 591, 12002 (2020).

Eriksson, T., Börjesson, J. & Tjerneld, F., Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose. Enzyme Microb. Technol., 31(3): 353–364 (2002).

Fatriasari, W., Nugroho Adi, D. T., Laksana, R. P. B., Fajriutami, T., Raniya, R., Ghozali, M., & Hermiati, E., The effect of amphipilic lignin derivatives addition on enzymatic hydrolysis performance of kraft pulp from sorghum bagasse. in IOP Conference Series: Earth and Environmental Science, 141(1) (2018).

Fatriasari, W., Nurhamzah, F., Raniya, R., Laksana, R.P.B., Anita, S.H., Iswanto, A.H., Hermiati, E., Enzymatic Hydrolysis Performance of Biomass by Addition of Lignin Based Biosurfactant. J. Korean Wood Sci. Technol., 48(5): 651–665 (2020).

Fatriasari, W., Hamzah, F. N., Pratomo, B. I., Fajriutami, T., Ermawar, R. A., Falah, F., Laksana, R. P. B., Ghozali, M., Iswanto, A. H., Hermiati, E., & Winarni, I., Optimizing the Synthesis of Lignin Derivatives from Acacia mangium to Improve the Enzymatic Hydrolysis of Kraft Pulp Sorghum Bagasse. Int. J. Renew. Energy Dev., 9(2): 227–235 (2020).

Winarni, I., Koda, K., Waluyo, T.K., Pari, G., Uraki, Y., Enzymatic saccarification of soda pulp from sago starch waste using sago lignin-based amphipatic derivatives. J. Wood Chem. Technol., 34(3): 157–168 (2014).

Uraki, Y, Koda, K, Yamada, T, Oikawa. C, Aso, T., in ACS Symposium Series Book, 243–254. (2012).

Wei, L., Shrestha, A., Tu, M. & Adhikari, S., Effects of surfactant on biochemical and hydrothermal conversion of softwood hemicellulose to ethanol and furan derivatives. Process Biochem., 46(9): 1785–1792 (2011).

Liu, J., Shi, J., Li, J., Yuan, X., & Liu, C., Effect of surfactant on ethanol production from rice straw by simultaneous saccharification and fermentation. in International Conference on Electronics, Communications and Control (ICECC), (2011).

Lee, W.G., Lee, J.S., Lee, J.P., Shin, C.S., Kim, M.S., & Park, S. C., Effect of surfactant on ethanol fermentation using glucose and cellulosic hydrolyzates. Biotechnol. Lett., 18(3): 299–304 (1996).

Laouar, L., Muligan, B. J. & Lowe, K. C., Yeast permeabilization with surfactants. Biotechnol. Lett., 14(8): 719–720 (1992).

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