Penentuan Kandungan Antioksidan Total pada Infusa Selada Hijau (Lactuca sativa L.) Hidroponik dan Konvensional secara Spektrofotometri dengan Modified Phenantroline Method (MPM)

Authors

  • Yefrida Yefrida Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang, Sumatra Barat, Indonesia https://orcid.org/0000-0002-1212-2422
  • Refilda Refilda Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang, Sumatra Barat, Indonesia
  • Nofi Hamidah Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang, Sumatra Barat, Indonesia
  • Widuri Rosman Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang, Sumatra Barat, Indonesia

DOI:

https://doi.org/10.25077/jrk.v13i1.492

Keywords:

antioxidant, MPM method, green lettuce, hydroponics

Abstract

Green lettuce is a vegetable that is widely consumed in Indonesia. These vegetables come from conventional and hydroponic farming. This study aims to compare the antioxidant content of these two types of vegetables. This vegetable is extracted by the infundation method. The extract obtained was determined for its antioxidant content by the MPM method. This method was validated by using parameter LoD, LoQ, linearity, precision and accuracy. The results showed that this method was valid for determining the total antioxidant content in conventional and hydroponic green lettuce samples. The total antioxidant content in conventional and hydroponic green lettuce samples was 2.68 ± 0.65 mg aa/g FW and 1.67 ± 0.40 mg AA/g FW, respectively. T test stated that the antioxidant content of the two samples was significantly different.

Author Biography

Yefrida Yefrida, Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Padang, Sumatra Barat

Scopus Profile:

https://www.scopus.com/authid/detail.uri?authorId=57191636458

References

Guo, H., Yuan, Q., Fu, Y., Liu, W., Su, Y. H., Liu, H., Wu, C. Y., et al., Extraction optimization and effects of extraction methods on the chemical structures and antioxidant activities of polysaccharides from snow chrysanthemum (Coreopsis tinctoria). Polymers (Basel)., 11(2): 1–21 (2019).

Nurhasnawati, H., Sundu, R., Sapri., Supriningrum, R., Kuspradini, H. & Arung, E. T., Antioxidant activity, total phenolic and flavonoid content of several indigenous species of ferns in East Kalimantan, Indonesia. Biodiversitas, 20(2): 576–580 (2019).

Yefrida., Ulfaningsih, M. & Loekman, U., Validasi metoda penentuan antioksidan total (dihitung sebagai asam sitrat) dalam sampel jeruk secara spektrofotometri dengan menggunakan oksidator FeCl3 dan pengompleks orto-fenantrolin. J. Ris. Kim., 7(2): 186–193 (2014).

Moelyono, M. W., Rochjana, A. U. H., Diantini, A., Mus, I., Sumiwi, S. A., Iskandar, Y. & Susilawati, Y., Aktivitas antioksidan daun Iler Plectranthus scutellarioides (L.) R. Br. J. Farm. Indones., 8(1): 271–276 (2016).

Masduki, A., Hidroponik sebagai sarana pemanfaatan lahan sempit di Dusun Randubelang, Bangunharjo, Sewon, Bantul. J. Pemberdaya., 1(2): 185–192 (2017).

Zapata-Vahos, I. C., Rojas-Rodas, F., David, D., Gutierrez-Monsalve, J. A. & Castro-Restrepo, D., Comparison of antioxidant contents of green and red leaf lettuce cultivated in hydroponic systems in greenhouses and conventional soil cultivation. Rev. Fac. Nac. Agron. Medellin, 73(1): 9077–9088 (2020).

Lei, C. & Engeseth, N. J., Comparison of growth characteristics, functional qualities, and texture of hydroponically grown and soil-grown lettuce. LWT, 150: 111931 (2021).

Strzemski, M., Dresler, S., Sowa, I., Czubacka, A., Agacka-Mołdoch, M., Płachno, B. J., Granica, S., et al., The impact of different cultivation systems on the content of selected secondary metabolites and antioxidant activity of Carlina acaulis plant material. Molecules, 25(1): 1–14 (2020).

Yefrida., Suyani, H., Aziz, H. & Efdi, M., Comparison of iron reduction methods on the determination of antioxidants content in vegetables sample. Orient. J. Chem., 34(5): 2418–2424 (2018).

Yefrida., Suryani, H., Alif, A., Azis, H. & Efdi, M., Modification of phenanthroline method to determine antioxidant content in tropical fruits methanolic extract. Res. J. Chem. Environ., 22(4): 28–35 (2018).

Yefrida, Y., Suyani, H., Aziz, H. & Efdi, M., Validasi metode MPM untuk penentuan kandungan antioksidan dalam sampel herbal serta perbandingannya dengan metode PM, FRAP dan DPPH. J. Ris. Kim., 11(1): 24–34 (2020).

Vishalli., Kaur, R., Raina, K. K. & Dharamvir, K., Investigation on single walled carbon nanotube thin films deposited by langmuir blodgett method. in AIP Conference Proceedings, 1661: 1–38 (2015).

Warjoto, R. E., Barus, T. & Mulyawan, J., Pengaruh media tanam hidroponik terhadap pertumbuhan bayam (Amaranthus sp.) dan selada (Lactuca sativa). J. Penelit. Pertan. Terap., 20(2): 118–125 (2020).

Mishra, P., Singh, U., Pandey, C., Mishra, P. & Pandey, G., Application of student’s t-test, analysis of variance, and covariance. Ann. Card. Anaesth., 22(4): 407 (2019).

Downloads

Published

2022-03-13

How to Cite

Yefrida, Y., Refilda, R., Hamidah, N., & Rosman, W. (2022). Penentuan Kandungan Antioksidan Total pada Infusa Selada Hijau (Lactuca sativa L.) Hidroponik dan Konvensional secara Spektrofotometri dengan Modified Phenantroline Method (MPM). Jurnal Riset Kimia, 13(1), 122–129. https://doi.org/10.25077/jrk.v13i1.492

Issue

Vol. 13 No. 1 (2022): March

Section

Articles

Citation Check

License

Please find the rights and licenses in Jurnal Riset Kimia (J. Ris. Kim). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.

1. License

The use the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License. 

2. Author(s)' Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights

Under the Creative Commons license, the journal permits users to copy, distribute, and display the material for any purpose. Users will also need to attribute authors and J. Ris. Kim on distributing works in the journal and other media of publications.

4. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

  • Copyright and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in own future works, including lectures and books,
  • The right to reproduce the article for own purposes,
  • The right to self-archive the article,
  • The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

5. Co-Authorship

If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. J. Ris. Kim will not be held liable for anything that may arise due to the author(s) internal dispute. J. Ris. Kim will only communicate with the corresponding author.