IN VITRO ANTIANGIOGENESIS ACTIVITY OF STANDARDIZED EXTRACTS OF Piper sarmentosum Roxb
ThisÂ studyÂ wasÂ undertakenÂ toÂ investigateÂ theÂ antiangiogenesisÂ activityÂ ofÂ standardizedÂ extracts/fractionsÂ ofÂ theÂ leafÂ ofÂ PiperÂ sarmentosum,Â usingÂ ratÂ aortaÂ model.Â TheÂ pulverizedÂ leafÂ wasÂ extractedÂ sequentiallyÂ andÂ methanolÂ extractÂ wasÂ furtherÂ fractionatedÂ withÂ hexane,Â chloroformÂ andÂ ethylacetate.Â BothÂ extractsÂ andÂ fractionsÂ wereÂ standardizedÂ byÂ reverseÂ phaseÂ HPLCÂ withÂ UVÂ detectionÂ atÂ 260Â nm,Â usingÂ twoÂ markers,Â sarmentineÂ andÂ sarmentosine.Â ChloroformÂ andÂ methanolÂ extractsÂ haveÂ exhibitedÂ antiangiogenesisÂ activityÂ ofÂ 100%Â andÂ 20%Â respectively.Â AntiangiogenesisÂ activityÂ ofÂ hexaneÂ andÂ chloroformÂ fractionsÂ wasÂ foundÂ toÂ beÂ 10%Â andÂ 90%Â respectively,Â whileÂ ethylacetateÂ fractionÂ wasÂ foundÂ toÂ beÂ inactive.Â TheÂ analysisÂ ofÂ mostÂ activeÂ extractÂ andÂ fractionÂ hasÂ exhibitedÂ differentÂ profileÂ byÂ HPLCÂ onÂ theÂ basisÂ ofÂ amides.Â ThisÂ studyÂ indicatesÂ thatÂ chloroformÂ extractÂ andÂ fractionÂ haveÂ promisingÂ antiangiogenesisÂ activityÂ andÂ haveÂ potentialÂ forÂ diseasesÂ involvingÂ angiogenesis.
KeywordsÂ :Â antiangiogenesisÂ activity,Â PiperÂ sarmentosumÂ Roxb.
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