Antibacterial Edible Coating from Mandarin Orange Peel (Citrus reticulata) and Moringa Leaf (Moringa oleifera) Extract for Fish Preservation

Authors

  • Maulidan Firdaus Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia https://orcid.org/0000-0001-8813-3649
  • Desy Nila Rahmana Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia
  • Diah Fitri Carolina Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia
  • Nisrina Rahma Firdausi Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia
  • Zulfaa Afiifah Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia
  • Berlian Ayu Rismawati Sugiarto Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Indonesia

DOI:

https://doi.org/10.25077/jrk.v14i1.563

Keywords:

Antibacterial, Edible coating, Moringa leaf, pectin, preservatives

Abstract

Approximately 10% of total fish production is wasted due to decomposition. Excessive formalin use in fish preservation can be potentially lethal. One of the natural preservatives is edible coating, which can be prepared from natural ingredients such as mandarin orange peel (Citrus reticulata) pectin and Moringa leaves (Moringa oleifera) which contain antibacterial compounds. The purpose of this research was to examine the antibacterial influence of edible coatings made from pectin of mandarin orange peel with the addition of Moringa leaf extract and to determine its effect on the freshness of nile tilapia (Oreochromis niloticus). Moringa leaf extraction was performed using the Soxhlet extraction method with 96% ethanol at 60–80 °C, while the mandarin orange peel was isolated by reflux using 1% HCl with a pH of 1.5 for 4 hours at 90 °C. Edible coating was synthesized from mandarin orange peel pectin and carboxymethyl cellulose (CMC) which was homogenized with distilled water, and glycerol was added as a plasticizer. Edible coatings were prepared with various concentrations of 0%, 50%, and 100% Moringa leaf extract. Nile tilapia with the addition of edible coatings experienced slower decay, especially in the variations of 100% Moringa leaf extract. This was evidenced by the quantitative test through the Total Plate Count (TPC) test which still did not exceed the limit of >5.6 log CFU/g.

References

Tran, N., Rodriguez, U-P., Chan, C.Y., Phillips, M.J., Mohan, C.V., Henriksson, P.J.G., Koeshendrajana, S., Suri, S., & Hall, S. Indonesian aquaculture futures: an analysis of fish supply and demand in Indonesia to 2030 and role of aquaculture using the Asia fish model, Mar. Policy, 79: 25-32 (2017).

Socaciu, M. I., Semeniuc, C. A., & Vodnar, D. C. Edible films and coatings for fresh fish packaging: focus on quality changes and shelf-life extension, Coatings, 8: 366-385 (2018).

Dehghani, S., Hosseini, S. V., & Regenstein, J. M. Edible films and coatings in seafood preservation: a review, Food Chem., 240: 505-513 (2018).

Sanyal, S., Sinha, K., Saha, S., & Banerjee, S. Formalin in fish trading: an inefficient practice for sustaining fish quality, Fish. Aquatic Life, 25: 43-50 (2017).

Mei, J., Ma, X., & Xie, J. Review on natural preservatives for extending fish shelf life, Foods, 8: 490 (2019).

Sucharitha, K. V., Beulah, A. M., & Ravikiran, K. Effect of chitosan coating on storage stability of tomatoes (Lycopersicon esculentum Mill), Int. Food Res. J., 25: 93-99 (2018).

Sayyari, Z., Rabani, M., Farahmandfar, R., Kenari, R.E., & Nadoshan, R.M. The effect of nanocomposite edible coating enriched with Foeniculum vulgare essential oil on the shelf life of Oncorhynchus mykiss fish fillets during the storage, J. Aquatic Food Prod. Technol., 30: 579-595 (2021).

Suhag, R., Kumar, N., Petkoska, A. T., & Upadhyay, A. Film formation and deposition methods of edible coating on food products: A review, Food Res. Int., 136: 109582 (2020).

Kantifedaki, A., Kachrimanidou, V., Mallouchos, A., Papanikolaou, S., & Koutinas, A. A. Orange processing waste valorisation for the production of bio-based pigments using the fungal strains Monascus purpureus and Penicillium purpurogenum, J. Clean. Prod., 185: 882-890 (2018).

Firdaus, M., Rahmana, D.N., Carolina, D.F., Firdausi, N.R., Afiifah, Z., & Sugiarto, B.A.R. Potential applications of antibacterial compounds in edible coating as fish preservative, Nat. Sci. Adv. Technol. Edu., 30: 45 –469 (2021).

Nurhaeni, Khairuddin, & Rosihan, R. Efficiency of SS400 steel corrosion bioinhibitor from moringa leaf (Moringa oleifera L.) extract in nacl solution medium, J. Phys. Conf. Ser., 1763: 1-5 (2021).

Alara, O. R., Abdurahman, N. H., & Ukaegbu, C. I. Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity, J. Appl. Res. Med. Arom. Plants, 11: 12-17 (2018).

Sugihartini, N., Sari, D. E. M., Bachri, M. S., & Yuliani, S. The Amount of β-carotene, total phenolic and total flavonoid of ethanol extract of leaf Moringa oleifera with variation concentration of solvent, Adv. Health Sci. Res., 18: 110–114 (2019).

Gopalasatheeskumar, K. Significant role of soxhlet extraction process in phytochemical research, Mintage J. Pharm. Med. Sci., 7: 43-47 (2018).

Oladeji, O. S., Odelade, K. A., & Oloke, J. K. Phytochemical screening and antimicrobial investigation of Moringa oleifera leaf extracts, African J. Sci., Technol, Innov. Dev., 12: 79-84 (2020).

Setyawan, H. Y., Sukardi, S., & Nareswari, B. F. The phytochemical potential of Averrhoa bilimbi–a review, IOP Conf. Ser. Earth Environ. Sci., 733: 1-7 (2021).

Paul, T. J., Taylor, T. A., & Santosh, A. B. R. The potential of saponin from Jamaica’s Blighia sapida (ackee) as a substitute for sodium lauryl sulphate in toothpaste. Med. Hypotheses, 137: 109555 (2020).

El Aziz, M. M. A., Ashour, A. S., & Melad, A. S. G. A Review on saponins from medicinal plants: chemistry, isolation, and determination. J. Nanomed. Res., 8: 282-288 (2019).

Muniyan, A., Ravi, K., Mohan, U., & Panchamoorthy, R. Characterization and in vitro antibacterial activity of saponin-conjugated silver nanoparticles against bacteria that cause burn wound infection, World J. Microbiol. Biotechnol., 33: 1-12 (2017).

Rahayu, Y.C., Sabir, A., & Setyorini, D. Antibacterial activity of red dragon fruit extract (Hylocereus polyrhizus) on Streptococcus mutans, Int. J. Appl. Pharm., 11: 60–63 (2019).

Twinomuhwezi, H., Godswill, A. C., & Kahunde, D. Extraction and characterization of pectin from orange (Citrus sinensis), lemon (Citrus limon) and tangerine (Citrus tangerina). Am. J. Phys. Sci., 1: 17-30 (2020).

Nandiyanto, A.B.D. Oktiani, R., & Ragadhita, R. How to read and interpret FTIR Spectroscopy of organic material, Indones. J. Sci. Technol., 4: 97-118 (2019).

Kpodo, F. M., Agbenorhevi, J. K., Alba, K., Bingham, R. J., Oduro, I. N., Morris, G. A., & Kontogiorgos, V. Pectin isolation and characterization from six okra genotype, Food Hydrocoll., 72: 323-330 (2017).

Wenno, M. R., Kaya, A. O. W., Lewerissa, S., Wattimena, M. L., Sormin, R. B. D., & Nanlohy, E. E. E. M. Utilization of garlic as traditional fish handling in Molluccas Islands: case study on layang fish (Decapterus macrosoma, B.), IOP Conf. Ser. Earth Environ. Sci., 797: 012018 (2021).

Fauziah, S., Efendi, F., Nugraheni, P. S., Murwantoko, Budhijanto, & Budhijanto, W., Kinetic study of fish decay inhibition by application of modified chitosan as preservative agent. AIP Conf. Proceed., 2085: 020011 (2019).

Downloads

Published

2023-03-24

How to Cite

Firdaus, M., Rahmana, D. N., Carolina, D. F. ., Firdausi, N. R. ., Afiifah, Z. ., & Sugiarto, B. A. R. . (2023). Antibacterial Edible Coating from Mandarin Orange Peel (Citrus reticulata) and Moringa Leaf (Moringa oleifera) Extract for Fish Preservation. Jurnal Riset Kimia, 14(1), 61–69. https://doi.org/10.25077/jrk.v14i1.563

Issue

Section

Articles

Citation Check