Komponen Kimia Minyak Atsiri yang Diisolasi dari Daun Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) dan Potensi Antibakteri serta Toksisitasnya

Authors

  • Suryati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, West Sumatra, Indonesia
  • Thaharah Alifa Aziz Yenuuar Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia
  • Shafinna Hana Fadhia Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia
  • Rahmi Vika Ulia Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia
  • Miftahul Melsya Salsabilla Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia
  • Bustanul Arifin Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Indonesia

DOI:

https://doi.org/10.25077/jrk.v14i1.583

Keywords:

Syzygium myrtifolium Walp, essential oil, antibacterial, Toxicity

Abstract

Pucuk merah (Syzygium myrtifolium Walp.) is an ornamental plant from the Myrtaceae family. Several phytochemical tests from previous researchers reported that pucuk merah contains secondary metabolites that have benefits such as antioxidants, antibacterials, and anti-cancer. In addition, the distinctive aroma produced from pucuk merah  leaves indicates the presence of essential oils whose bioactivity can be studied. Still, there has been no report on this plant's chemical components or bioactivity. Therefore, this study aims to determine the content of chemical components of pucuk merah  essential oil and their bioactivity as antibacterial and toxicity. The isolation of essential oils was carried out by the hydro distillation method. The chemical components of essential oils were analyzed using the Gas Chromatography-Mass Spectrometry (GC-MS) method. GC-MS results showed the presence of 42 compounds with four main compounds, namely caryophyllene (23.45%), 3-carene (15.67%), α-terpineol (10.74%), and α-pinene (5.98%) ). Antibacterial test of pucuk merah  essential oil using the disc diffusion method showed strong activity against Staphylococcus aureus and Escherichia coli with inhibition zones of 16.15 ± 2.03 and 16.13 ± 1.74 mm at 100% concentration. The results of the toxicity test of pucuk merah  essential oil using the BSLT (Brine Shrimp Lethality Test) method on Artemia salina Leach shrimp larvae showed strong toxic properties with an LC50 value of 3.99 µg/mL.

Author Biography

Suryati, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, West Sumatra

Scopus Profile:

https://www.scopus.com/authid/detail.uri?authorId=57205311075

References

Widjaja, E., Rahayuningsih, Y., Rahajoe, J., Ubaidillah, R., Maryanto, I., Walujo, E. & Semiadi, G., Kekinian Keanekaragaman Hayati Indonesia. LIPI Press, (2014).

Haryati, N. A., Saleh, C. & Erwin., Uji Toksisitas dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) Terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli. J. Kim. Mulawarman, 13: 35–40 (2015).

Santoni, A., Darwis, D. & Syahri, S., Isolasi Antosianin dari Buah Pucuk Merah (syzygium campanulatum korth.) Serta Pengujian Antioksidan dan Aplikasi sebagai Pewarna Alami. Pros. Semirata FMIPA Univ. Lampung, 1(1): 1–10 (2013).

Zulfikar, E., Wiendarlina, I. Y. & Wardatun, S., Penelusuran Potensi Antikanker Daun Pucuk Merah (Syzygium campanulatum Korth) dengan Metode Brine Shrimps Lethality Test (BSLT). Universitas Pakuan. Bogor, (2015).

Jena, S., Ray, A., Sahoo, A., Das, P. K., Dash, K. T., Kar, S. K., Nayak, S., et al., Chemical Composition and Biological Activities of Leaf Essential Oil of Syzygium myrtifolium from Eastern India. J. Essent. Oil-Bearing Plants, 24(3): 582–595 (2021).

Juwita, R., Chairul, S. & Saibun, S., Uji Aktivitas Antihiperurisemia dari Daun Hijau Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) Terhadap Mencit Jantan (Mus Musculus). J. At., 2(1): 162–168 (2017).

Aisha, A. F. A., Ismail, Z., Abu-Salah, K. M., Siddiqui, J. M., Ghafar, G. & Abdul Majid, A. M. S., Syzygium Campanulatum Korth Methanolic Extract Inhibits Angiogenesis and Tumor Growth In Nude Mice. BMC Complement. Altern. Med., 13: 168 (2013).

Memon, A. H., Ismail, Z., Aisha, A. F. A., Al-suede, F. S. R., Shahrul, M., Hamil, R., Hashim, S., et al., Isolation , Characterization , Crystal Structure Elucidation , and Anticancer Study of Dimethyl Cardamonin , Isolated from Syzygium campanulatum Korth. Evidence-Based Complement. Altern. Med., Vol. 2014: 11 (2014).

Sharifi-Rad, J., Sureda, A., Tenore, G. C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., et al., Biological activities of essential oils: From plant chemoecology to traditional healing systems. Molecules, 22(1): (2017).

Suryati., Santon, A., Arifin, B., Ferdinal, N., Salim, E., Amelia, A., Zein, L., et al., Analysis of Chemical Content, Cytotoxic and Anti-Bacterial Activity Essential Oil of Lantana Camara Linn Leaves from Various Regions. Molekul, 17(2): 156–164 (2022).

Baharun, K., Rukmi, I., Lunggani, A. T. & Fachriyah, E., Daya Antibakteri Berbagai Konsentrasi Minyak Atsiri Rimpang Temu Hitam (Curcuma Aeruginosa Roxb.) Terhadap Bacillus Subtilis dan Staphyloccus Aureus Secara In Vitro. J. Biol., 2(4): 16–24 (2013).

Suryati., Aziz, E. D., Efdi, M., Wahyuni, F. S. & Hefni, D., Analysis of The Essential Oil from Lantana camara Leaves and Its Cytotoxic Potential Against T-47D Breast Cancer Cells. J. Risat Kim., 12: 1–9 (2021).

Suryati., Ibrahim, S. & Aziz, E. D., Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Rengas (Gluta renghas L.). J. Kim. Unand, 6(2303): 37–41 (2017).

Suryati., Hefni, D., Wahyuni, F. S. & Dachriyanus., The cytotoxicity study of lantana camara Linn essential oil on HeLa cancer cells line. Pharmacogn. J., 13(6): 1498–1501 (2021).

Aziz, E. D., Isolasi Triterpenoid dan Minyak Atsiri dari Daun Tumbuhan Lantana camara Linn serta Aktivitas Sitotoksiknya. Universitas Andalas, (2020).

Delnavazi, M. R., Saiyarsarai, P., Jafari-Nodooshan, S., Khanavi, M., Tavakoli, S., Hadavinia, H. & Yassa, N., Cytotoxic flavonoids from the aerial parts of Stachys lavandulifolia vahl. Pharm. Sci., 24(4): 332–339 (2018).

Sánchez Perera, L. M., Mancebo Dorvigny, B. & Regalado Veloz, A. I., Inhibition of seed germination, toxicity on Artemia salina and phytochemical prospecting with from Cuban plants as indicator of antitumor activity. Maced. Pharm. Bull., 63(02): 29–36 (2018).

Muharni., Fitrya. & Farida, S., Antibacterial assay of ethanolic extract musi tribe medicinal plant in Musi Banyuasin, South Sumatera. J. Kefarmasian Indones., 7(2): 127–135 (2017).

Anggia, Fela Tri. Yuharmen, N. B., Kenanga ( Cananga odorata ( Lam .) Hook . f & Thoms ) Cara Konvensional dan Microwave serta Uji Aktivitas Antibakteri dan Antioksidan. 1(2): 344–351 (2014).

R. Hamidi, M., Jovanova, B. & Kadifkova Panovska, T., Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model. Maced. Pharm. Bull., 60(01): 9–18 (2014).

Bello, O. A., Ayanda, O. I., Aworunse, O. S. & Olukanmi, B. I., Terpenoids as Cytotoxic Compounds: A Perspective. 1(2): 166–176 (2018).

Downloads

Published

2023-03-24

How to Cite

Suryati, Yenuuar, T. A. A. ., Fadhia, S. H. ., Ulia, R. V., Salsabilla , M. M. ., & Arifin, B. (2023). Komponen Kimia Minyak Atsiri yang Diisolasi dari Daun Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) dan Potensi Antibakteri serta Toksisitasnya. Jurnal Riset Kimia, 14(1), 70–80. https://doi.org/10.25077/jrk.v14i1.583

Issue

Vol. 14 No. 1 (2023): March

Section

Articles

Citation Check

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Please find the rights and licenses in Jurnal Riset Kimia (J. Ris. Kim). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.

1. License

The use the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution 4.0 International License. 

2. Author(s)' Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights

Under the Creative Commons license, the journal permits users to copy, distribute, and display the material for any purpose. Users will also need to attribute authors and J. Ris. Kim on distributing works in the journal and other media of publications.

4. Rights of Authors

Authors retain all their rights to the published works, such as (but not limited to) the following rights;

  • Copyright and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in own future works, including lectures and books,
  • The right to reproduce the article for own purposes,
  • The right to self-archive the article,
  • The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.

5. Co-Authorship

If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. J. Ris. Kim will not be held liable for anything that may arise due to the author(s) internal dispute. J. Ris. Kim will only communicate with the corresponding author.