Fitokimia, Kadar Fenolik Total, dan Flavonoid Total serta Aktivitas Antioksidan Ekstrak n-Heksana Rimpang Temu Hitam (Curcuma aeruginosa Roxb)

Authors

  • Auliya Ilmiawati Department of Chemistry Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor, Indonesia
  • Ika Resmeiliana Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • Ishika Jauza Nasywa Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • Fadia Fahira Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • Nafisa Muthia Wafa Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • M. Raffi Rayandhika Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • Andi Thaariq Muhammad Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • Arini Septianti Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia
  • Hafizah Fatunisa Program Studi Analisis Kimia, Sekolah Vokasi IPB, Kota Bogor, Indonesia

DOI:

https://doi.org/10.25077/jrk.v14i2.599

Keywords:

Antioxidant, Black turmeric, Curcuma aeruginosa, Flavonoids, Phenol

Abstract

The rhizome of black turmeric (Curcuma aeruginosa) is a plant rich in benefits and has been used as a herbal medicine to treat various health problems. This study aims to obtain the n-hexane extract of black turmeric rhizome and perform phytochemical tests, determine total phenolic and total flavonoid levels, as well antioxidant tests using the DPPH method. Extraction of n-hexane from black turmeric rhizome obtained yield of 4.88%, total phenolic content of 3.01 mg QE/g extract and total flavonoids of 6.31 mg QE/g extract. The phytochemical test obtained positive results for the presence of alkaloids and flavonoids; negative tannins, steroids/triterpenoids and saponins. Antioxidant tests using the DPPH method obtained the percentage of DPPH capture ranging from 76-78%, with the largest percentage value obtained when the extract concentration was 125 ppm, which was 78.02%. This shows that black turmeric n-hexane extract has weak antioxidant activity.

Author Biography

Auliya Ilmiawati, Department of Chemistry Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor

References

Marliani, L., Sukmawati, I. K., Juanda, D., Anjani, E. & Anggraeni, I., Penapisan Fitokimia, Kadar Kurkuminoid dan Aktivitas Antibakteri Temu Hitam (Curcuma aeruginosa (Christm) Roscoe.), Temu Putih (Curcuma zedoaria Roxb.) dan Temulawak (Curcuma xanthorrhiza Roxb.). Herb-Medicine J., 4(1): 57–64 (2021).

Suphrom, N., Pumthong, G., Khorana, N., Waranuch, N., Limpeanchob, N. & Ingkaninan, K., Anti-androgenic effect of sesquiterpenes isolated from the rhizomes of Curcuma aeruginosa Roxb. Fitoterapia, 83(5): 864–871 (2012).

Hossain, C. F., Al-Amin, M., Sayem, A. S. M., Siragee, I. H., Tunan, A. M., Hassan, F., Kabir, M. M., et al., Antinociceptive principle from Curcuma aeruginosa. BMC Complement. Altern. Med., 15(197): 1–7 (2015).

Fitria, R., Seno, D. S. H., Priosoeryanto, B. P., Hartanti. & Nurcholis, W., Volatile compound profiles and cytotoxicity in essential oils from rhizome of Curcuma aeruginosa and Curcuma zanthorrhiza. Biodiversitas, 20(10): 2943–2948 (2019).

Vanda, H., Parindra, R., Hambal, M. & Athaillah, F., Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro. E3S Web Conf., 151(01046): 1–3 (2020).

Reanmongkol, W., Subhadhirasakul, S., Khaisombat, N., Fuengnawakit, P., Jantasila, S. & Khamjun, A., Investigation the antinociceptive, antipyretic and anti-inflammatory activities of Curcuma aeruginosa Roxb. extracts in experimental animals. Songklanakarin J. Sci. Technol., 28(5): 999–1008 (2006).

Simoh, S., Shin, S. Y., Rahim, F. A., Ahmad, M. A. & Zainal, A., Comparative analysis of metabolites and antioxidant potentials from different plant parts of Curcuma aeruginosa Roxb. Sains Malaysiana, 47(12): 3031–3041 (2018).

Arsa, A. K. & Achmad, Z., Ekstraksi minyak atsiri dari rimpang temu ireng (Curcuma aeruginosa Rox) Dengan Pelarut etanol dan n-heksana. J. Teknol. Technoscientia, 13(1): 83–94 (2020).

Maesaroh, K., Kurnia, D. & Al Anshori, J., Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin. Chim. Nat. Acta, 6(2): 93–100 (2018).

Batubara, I., Zahra, U., Darusman, L. K. & Maddu, A., Minyak atsiri daun Zingiberaceae sebagai antioksidan dan antiglikasi. Indones. J. Essent. Oil, 1(1): 44–52 (2016).

Sumazian, Y., Syahida, A., Hakiman, M. & Maziah, M., Antioxidant activities, flavonoids, ascorbic acid and phenolic contents of Malaysian vegetables. J. Med. Plants Res., 4(10): 881–890 (2010).

Shaikh, J. R. & Patil, M., Qualitative tests for preliminary phytochemical screening: An overview. Int. J. Chem. Stud., 8(2): 603–608 (2020).

Aghajani, Z. & Engashte-Vahed, A. A., Composition of volatile oils and antioxidant activity of water extracts of leaves of Ziziphus jujuba mill. From central Iran. Malaysian Appl. Biol., 44(2): 93–98 (2015).

Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P. & Vidal, N., Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem., 97: 654–660 (2006).

Shimada, K., Fujikawa, K., Yahara, K. & Nakamura, T., Antioxidative Properties of Xanthan on the Autoxidation of Soybean Oil in Cyclodextrin Emulsion. J. Agric Food chem, 40: 945–948 (1992).

Zulfiah, Z., Megawati, M., Herman, H., H. Ambo Lau, S., Hasyim, M. F., Murniati, M., Roosevelt, A., et al., Uji Toksisitas Ekstrak Rimpang Temu Hitam (Curcuma aeruginosa Roxb.) Terhadap Larva Udang (Artemia salina Leach) dengan Metode Brine Shrimp Lethality Test (BSLT). J. Farm. Sandi Karsa, 6(1): 44–49 (2020).

Mustariani, B. A. A., Izuddin, A., Hasyim, D. M. & Batubara, I., Uji Toksisitas dan Aktivitas Antimakan Ekstrak Rimpang Temu Hitam (curcuma aeruginosa roxb). J. Farmasetis, 6(1): 1–8 (2017).

Nurcholis, W., Khumaida, N., Syukur, M. & Bintang, M., Analisis Kemiripan 20 Aksesi Temu Ireng (Curcuma aeruginosa Roxb.) Berdasarkan Warna Rimpang, Hasil Ekstrak, dan Kandungan Fitokimia. J. Agron. Indones. (Indonesian J. Agron., 44(3): 315–321 (2016).

Nugrahaningtyas, K. D., Matsjeh, S. & Wahyuni, T. D., Isolasi dan Identifikasi Senyawa Flavonoid dalam Rimpang Temu Ireng (Curcuma aeruginosa Roxb.). Biofarmasi, 3(1): 32–38 (2005).

Ahmad, A. R., Juwita, J., Ratulangi, S. A. D. & Malik, A., Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Pharm. Sci. Res., 2(1): 1–10 (2015).

Ford, L., Theodoridou, K., Sheldrake, G. N. & Walsh, P. J., A critical review of analytical methods used for the chemical characterisation and quantification of phlorotannin compounds in brown seaweeds. Phytochem. Anal., 30(6): 587–599 (2019).

Zuhri., Analisis Regresi Linier dan Korelasi menggunakan Pemrograman Visual Basic. J. Ilman J. Ilmu Manaj., 8(2): 42–50 (2020).

Sandrasari, D. A., Sabariman, M. & Azni, I. N., Determination of potential level of Indonesian rhizomes as an antioxidant based on phenolic compound and antioxidant activity. IOP Conf. Ser. Earth Environ. Sci., 383(012017): (2019).

Zahra, u., Kartika, y., Batubara, i., Darusman, l. K. & Maddu, A., Short Communication: Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent. Nusant. Biosci., 8(2): 221–225 (2016).

Al-reza, S. M., Rahman, A., Sattar, M. A., Rahman, M. O. & Fida, H. M., Essential oil composition and antioxidant activities of Curcuma aromatica Salisb . Food Chem. Toxicol., 48(6): 1757–1760 (2010).

Febriani, A. K. & Anam, K., Identifikasi Kumarin dan Pengaruh Jenis Pelarut terhadap Total Kumarin pada Ekstrak Buah Api-Api Putih (A . marina). Med. Sains, 7(4): 735–742 (2022).

Aris, M. & Adriana, A., Penentuan Kadar Total Flavonoid Dan Nilai SPF (Sun Protection Factor) Ekstrak Etanol Rimpang Temu Ireng (Curcuma Aeruginosa Roxb.) Secara Spektrofotometri Uv-Vis. J. Pharm. Sci., 12(2): 85–93 (2022).

Azizah, D. N., Kumolowati, E. & Faramayuda, F., Penetapan Kadar Flavonoid Metode AlCl3 pada Ekstrak Metanol Kulit Buah Kakao (Theobroma cacao L.) Dyah Nur Azizah, Endang Kumolowati, Fahrauk Faramayuda. KARTIKA J. Ilm. Farm., 2(2): 45–49 (2014).

Lee, J. H., Cho, S., Paik, H. D., Choi, C. W., Nam, K. T., Hwang, S. G. & Kim, S. K., Investigation on Antibacterial and Antioxidant Activities , Phenolic and Flavonoid Contents of Some Thai Edible Plants as an Alternative for Antibiotics. Asian Australas. J. Anim. Sci, 27(10): 1461–1468 (2014).

Wibawa, J. C., Arifin, M. Z. & Herawati, L., Mekanisme Vitamin C Menurunkan Stres Oksidatif Setelah Aktivitas Fisik. J. Sport Sci. Educ., 5(1): 57–63 (2020).

Zuliani, N. E. & Kusuma, I. W., Uji Aktivitas Antioksidan (Metode DPPH) Ekstrak Metanol dan Fraksi - Fraksinya dari Daun Rumput Knop (Hyptis capitata Jacq.). J. At., 04(1): 36–40 (2019).

Rahmawati, R., Muflihunna, A. & Sarif, L. M., Analisis Aktivitas Antioksidan Produk Sirup Buah Mengkudu (morinda citrifolia l.) dengan metode dpph. J. Fitofarmaka Indones., 2(2): 97–101 (2016).

Kaban, A. N., Daniel. & Saleh, C., Uji Fitokimia, Toksisitas dan Aktivitas Antioksidan Fraksi n-Heksana dan Etil Asetat Terhadap Ekstrak Jahe Merah (Zingiber officinale var. amarum.). J. Kim. Mulawarman, 14(1): 24–28 (2016).

Downloads

Published

2023-10-17

How to Cite

Ilmiawati, A. ., Resmeiliana, I. ., Ishika Jauza Nasywa, Fadia Fahira, Nafisa Muthia Wafa, M. Raffi Rayandhika, Andi Thaariq Muhammad, Arini Septianti, & Hafizah Fatunisa. (2023). Fitokimia, Kadar Fenolik Total, dan Flavonoid Total serta Aktivitas Antioksidan Ekstrak n-Heksana Rimpang Temu Hitam (Curcuma aeruginosa Roxb). Jurnal Riset Kimia, 14(2), 107–117. https://doi.org/10.25077/jrk.v14i2.599

Issue

Section

Articles

Citation Check