Antibacterial Activity against Gram-Positive and Gram-Negative Bacteria and Biolarvicide to Aedes aegypti from Cocok Bubu (Elatostema rostratum (Blume) Hassk) Leaves Extract


  • Lela Lailatul Khumaisah Department of Chemistry Faculty of Science and Technology, Universitas Muhammadiyah Sukabumi, Indonesia
  • Rika Ayuningtias Department of Chemistry Faculty of Science and Technology, Universitas Muhammadiyah Sukabumi, Indonesia
  • Salih Muharam Department of Chemistry Faculty of Science and Technology, Universitas Muhammadiyah Sukabumi, Indonesia
  • Reni Mulyani Department of Chemistry Faculty of Science and Technology, Universitas Muhammadiyah Sukabumi, Indonesia
  • Luthfi Awaliah Department of Chemistry Faculty of Science and Technology, Universitas Muhammadiyah Sukabumi, Indonesia



Neglected tropical disease (NTDs), Cocok bubu (Elatostema rostratum (Blume) Hassk), antibacterial, Elatostema rostratum, phytochemicals screening, toxicity, biolarvicide, antibacterial


Tropical diseases are infectious diseases that commonly occur in tropical climates. Infectious diseases are caused by bacteria, viruses, parasites, and are transmitted by several vectors. There are 8 neglected tropical diseases (NTDs) that have spread in Indonesia. Therefore, massive efforts are needed to overcome this disease. Active plant substances have long been popular in treating various diseases. Cocok Bubu (Elatostema rostratum (Blume) Hassk) is an endemic plant of Indonesia. Ethnobotanical studies of this plant are used to treat fever and diarrhea. But, there are no pharmacological studies on previous research because this species belongs to a limited distribution plant. However, Elatostema has a secondary metabolite with various pharmacological activities. This study aims to determine the activity of antibacterial and biolarvicides from Cocok bubu leaf extract. The extraction using the maceration method with acetone, phytochemicals screening, toxicity test using BSLT, antibacterial test with disc diffusion to Escherichia coli, Staphylococcus aureus, Streptococcus mutant, and Streptococcus sanguinis, and biolarvicide activity against Aedes aegypti. Based on the results, Cocok bubu leaf extract contains flavonoids, alkaloids, saponin, tannin, terpenoids, and steroids with a toxicity of 758.45 ppm. From antibacterial activity results, Cocok bubu exctract had the best inhibition zone against Staphylococcus aureus (10 – 18 mm). According to the biolarvicide test, it is effective as a biolarvicide to Aedes aegypti with LC50 51.099 ppm and LT50 of 5 h 43 min. This study shows that Cocok bubu was more effective for treating and preventing NTDs in Indonesia, especially dengue and chikungunya fever in the future.


Ruminem., Tandirogang, N., Bakhtiar, R., Rahayu, A. P. & Kadir, A., Modul Penyakit Tropis. (2020).

Howden, B. P., Giulieri, S. G., Wong Fok Lung, T., Baines, S. L., Sharkey, L. K., Lee, J. Y. H., Hachani, A., et al., Staphylococcus aureus host interactions and adaptation. Nat. Rev. Microbiol., 21(6): 380–395 (2023).

Javed, M. R., Salman, M., Tariq, A., Tawab, A., Zahoor, M. K., Naheed, S., Shahid, M., et al., Phthalate from Lactiplantibacillus plantarum. Molecules, 27: 7220 (2022).

Mutsaqof, A. A. N., Wiharto. & Suryani, E., Sistem Pakar Untuk Mendiagnosis Penyakit Infeksi Menggunakan Forward Chaining. J. Teknol. Inf. ITSmart, 4(1): 43–47 (2015).

Kementerian Kesehatan RI, B. K. dan P. P., Sejumlah Penyakit Tropis Ini Harus Diwaspadai. (2023).

Mackowiak, P. A., Chervenak, F. A. & Grünebaum, A., Defining Fever. Open Forum Infect. Dis., 8(6): (2021).

Uddin, Z., Emran, T. Bin., Dutta, M., Ullah, S. M. A., Hossain, S. & Rana, S., In vivo antidepressant , analgesic , anti-inflammatory activities , in vitro antioxidant and antibacterial potential of fractionated Elatostema papillosum Wed . extract. Pharma Innov., 8(1): 241–246 (2019).

Silalahi, M., Supriatna, J., Walujo, E. B. & Nisyawati., Local knowledge of medicinal plants in sub-ethnic Batak Simalungun of North Sumatra, Indonesia. Biodiversitas, 16(1): 44–54 (2015).

Yin, Z., Zhang, W. & Kang, W., Identification of Volatile Compounds of Elatostema duyunense by HS-SPME-GC-MS. Chem. Nat. Compd., 52(5): 928–929 (2016).

Nandwani, D., Calvo, J. A., Tenorio, J. & Felix Calvo and Lourdes Manglona., Medicinal plants and traditional knowledge in the. J. Appl. Biosci., 8: 323–330 (2008).

Upadhyay, A. K., Gogoi, R. & Mitra, P. K., Ethnobotany of the Genus Elatostema J.R. Forster & G. Forster (Urticaceae) Ashutosh. Ethnobot. Res. Appl., 21: 143–163 (2021).

Mariani, R., Suganda, A. G. & Sukandar, E. Y., Drug-drug interactions between griseofulvinand a new prenylated chalcone from elatostema parasiticum and its antibacterial activity nortriptylineat binding sites of bovine serum albumin. Pharmacologyonline, 2016(1): 1–6 (2016).

Mariani, R., Sukandar, E. Y. & Suganda, A. G., Antimicrobial activity from indonesian urticaceae. Int. J. Pharm. Pharm. Sci., 6(4): 191–193 (2014).

Mariani, R., Sukandar, E. Y. & Suganda, A. G., Antimicrobial activity of Elatostesma parasiticum. World J. Pharm. Sci., 3(6): 1083–1086 (2015).

Ali Reza, A. S. M., Hossain, M. S., Akhter, S., Rahman, M. R., Nasrin, M. S., Uddin, M. J., Sadik, G., et al., In vitro antioxidant and cholinesterase inhibitory activities of Elatostema papillosum leaves and correlation with their phytochemical profiles: A study relevant to the treatment of Alzheimer’s disease. BMC Complement. Altern. Med., 18(1): (2018).

Miyazawa, M., Utsumi, Y. & Kawata, J., Aroma-active compounds of Elatostema laetevirens and Elatostema umbellatum var. majus. J. Oleo Sci., 58(4): 163–169 (2009).

Farhadi, F., Khameneh, B., Iranshahi, M. & Iranshahy, M., Antibacterial activity of flavonoids and their structure–activity relationship: An update review. Phyther. Res., 33(1): 13–40 (2019).

Yan, Y., Li, X., Zhang, C., Lv, L., Gao, B. & Li, M., Research progress on antibacterial activities and mechanisms of natural alkaloids: A review. Antibiotics, 10(3): (2021).

Wuillda, A. C. J. D. S., Martins, R. C. C. & Costa, F. D. N., Larvicidal activity of secondary plant metabolites in aedes aegypti control: An overview of the previous 6 years. Nat. Prod. Commun., 14(7): (2019).

Adusei, S., Otchere, J. K., Oteng, P., Mensah, R. Q. & Tei-Mensah, E., Phytochemical analysis, antioxidant and metal chelating capacity of Tetrapleura tetraptera. Heliyon, 5(11): e02762 (2019).

Iqbal, E., Salim, K. A. & Lim, L. B. L., Phytochemical screening, total phenolics and antioxidant activities of bark and leaf extracts of Goniothalamus velutinus (Airy Shaw) from Brunei Darussalam. J. King Saud Univ. - Sci., 27(3): 224–232 (2015).

Puspa, O. E., Syahbanu, I. & Wibowo, M. A., Uji Fitokimia dan Toksisitas Minyak Atsiri Daun Pala ( Myristica fragans Houtt ) Dari Pulau Lemukutan. J. Kim. Khatulistiwa, 6(2): 1–6 (2017).

Balouiri, M., Sadiki, M. & Ibnsouda, S. K., Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal., 6(2): 71–79 (2016).

WHO., Guidelines for laboratory and field testing of mosquito larvicides. World Health Organization, (2005).

Meyer, B. N., Ferrigni, N. R., Putnam, J. E., Jacobsen, L. B., Nichols, D. E. & McLaughlin, J. L., Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med., 45(1): 31–34 (1982).

Purnama, A., Efektifitas Ekstrak dan Fraksi Daun dan Batang Canar Bokor (Smilax leucophylla Blume) sebagai Biolarvasida terhadap Larva Nyamuk Aedes aegypti. Universitas Muhammadiyah Sukabumi, (2018).

Rahmawati, F., Skrining Fitokimia, Fraksinasi dan Uji Antibakteri Ekstrak Daun Canar Bokor (Smilax leucophylla Blume) terhadap Escherechia coli. Universitas Muhammadiyah Sukabumi, (2018).

Rampadarath, S., Puchooa, D. & Ranghoo-Sanmukhiya, V. M., Antimicrobial, phytochemical and larvicidal properties of Jatropha multifida Linn. Asian Pac. J. Trop. Med., 7(S1): S380–S383 (2014).

Soni, N. & Dhiman, R. C., Larvicidal and antibacterial activity of aqueous leaf extract of Peepal (Ficus religiosa) synthesized nanoparticles. Parasite Epidemiol. Control, 11: e00166 (2020).

Greenwood, D., Resistance to antimicrobial agents: A personal view. J. Med. Microbiol., 47(9): 751–755 (1998).

Khameneh, B., Iranshahy, M., Soheili, V. & Fazly Bazzaz, B. S., Review on plant antimicrobials: A mechanistic viewpoint. Antimicrob. Resist. Infect. Control, 8(1): (2019).

Hurley, K. A., Santos, T. M. A., Nepomuceno, G. M., Huynh, V., Shaw, J. T. & Weibel, D. B., Targeting the Bacterial Division Protein FtsZ. J. Med. Chem., 59(15): 6975–6998 (2016).

Yusuf, A. J., Abdullahi, M. I., Aleku, G. A., Ibrahim, I. A. A., Alebiosu, C. O., Yahaya, M., Adamu, H. W., et al., Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla. J. Med. Plants Econ. Dev., 2(1): 1–5 (2018).

Dong, S., Yang, X., Zhao, L., Zhang, F., Hou, Z. & Xue, P., Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria. Ind. Crops Prod., 149: (2020).

Assaf, H., Nafady, A., Allam, A., Hamed, A. & Kamel, M., Phytochemistry and biological activity of family ‘Urticaceae’: a review (1957-2019). J. Adv. Biomed. Pharm. Sci., 3(3): 150–176 (2020).

Górniak, I., Bartoszewski, R. & Króliczewski, J., Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry Reviews, 18(1): (2019).

Mahizan, N. A., Yang, S., Moo, C.-L. & Song, A. A.-L., Terpene Derivatives as a Potential Agent against. Molecules, 24(2631): 1–21 (2019).

Shang, X. F., Morris-Natschke, S. L., Liu, Y. Q., Guo, X., Xu, X. S., Goto, M., Li, J. C., et al., Biologically active quinoline and quinazoline alkaloids part I. Med. Res. Rev., 38(3): 775–828 (2018).

Alvarez, M. R., Heralde, F. & Quiming, N., Screening for larvicidal activity of ethanolic and aqueous extracts of selected plants against Aedes aegypti and Aedes albopictus larvae. J. Coast. Life Med., 4(2): 143–147 (2016).

Colovic, M. B., Krstic, D. Z., Lazarevic-Pasti, T. D., Bondzic, A. M. & Vasic, V. M., Acetylcholinesterase Inhibitors: Pharmacology and Toxicology. Curr. Neuropharmacol., 11(3): 315–335 (2013).

Musau, J. K., James, M. M., Joseph, M. N., Mbaabu, M. & Stephen, G. K., Phytochemical composition and larvicidal properties of plants used for mosquito control in Kwale County, Kenya. Int. J Mosq. Res., 3(3): 12–17 (2016).

Fernandes, D. A., Barros, R. P. C., Teles, Y. C. F., Oliveira, L. H. G., Lima, J. B., Scotti, M. T., Nunes, F. C., et al., Larvicidal compounds extracted from helicteres velutina K. Schum (Sterculiaceae) evaluated against aedes aegypti L. Molecules, 24(12): (2019).

Geris, R., Ribeiro, P. R., Da Silva Brandão, M., Da Silva, H. H. G. & Da Silva, I. G., Bioactive natural products as potential candidates to control Aedes aegypti, the vector of dengue. Studies in Natural Products Chemistry, 37: Elsevier, (2012).

Mahdi, N., Ridha, M. R., Setiawan, D., Praristiya, M. R. S., Rahayu, N. & Atmaja, B. P., Bio-efficacy of Mangifera leaf extracts on mortality of Aedes aegypti and inhibition of egg hatching. Vet. World, 15(7): 1753–1758 (2022).

Mardiana., Supraptini. & Aminah, N. S., Datura metel Linnaeus sebagai Insektisida dan Larvasida Botaniserta Bahan Baku Obat Tradisional. Media Penelit. dan Pengemb. Kesehat., 19: (2009).

Andrade-Ochoa, S., Correa-Basurto, J., Rodríguez-Valdez, L. M., Sánchez-Torres, L. E., Nogueda-Torres, B. & Nevárez-Moorillón, G. V., In vitro and in silico studies of terpenes, terpenoids and related compounds with larvicidal and pupaecidal activity against Culex quinquefasciatus Say (Diptera: Culicidae). Chem. Cent. J., 12(1): 1–21 (2018).




How to Cite

Lela Lailatul Khumaisah, Rika Ayuningtias, Salih Muharam, Reni Mulyani, & Luthfi Awaliah. (2023). Antibacterial Activity against Gram-Positive and Gram-Negative Bacteria and Biolarvicide to Aedes aegypti from Cocok Bubu (Elatostema rostratum (Blume) Hassk) Leaves Extract. Jurnal Riset Kimia, 14(2), 158–166.




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