Analysis of Total Phenolics, Flavonoids, and Antioxidant Activity of Cashew Leaf Extract (Anacardium occidentale L.) with Varying Ethanol Concentrations

Analisis Total Fenolik, Flavonoid, dan Aktivitas Antioksidan Ekstrak Daun Jambu Mete (Anacardium occidentale L.) dengan Variasi Konsentrasi Etanol

Authors

  • Risma Kurniasari Department of Chemistry, Diponegoro University, Tembalang, Semarang, Indonesia
  • Meiny Suzery Department of Chemistry, Diponegoro University, Tembalang, Semarang, Indonesia
  • Bambang Cahyono Department of Chemistry, Diponegoro University, Tembalang, Semarang, Indonesia

DOI:

https://doi.org/10.25077/jrk.v15i2.735

Keywords:

anacardium occidentale, total phenolics, total flavonoids, antioxidant activity, ethanol extraction

Abstract

This study evaluated the effectiveness of various ethanol concentrations (50%, 70%, and 96%) in extracting bioactive compounds from cashew leaves (Anacardium occidentale). Parameters analyzed included total phenolics, total flavonoids, and antioxidant activity using the DPPH and H2O2 methods. The results showed that the 70% ethanol extract produced the highest total phenolic and flavonoid content as well as significant antioxidant activity compared to other ethanol concentrations. This study offers practical insights for pharmaceutical, cosmetic, and nutraceutical industries in selecting the optimal solvents for cashew leaf extraction. Additionally, the analysis showed that the 70% ethanol extract offered an optimal balance between extraction efficiency and antioxidant activity, making it the most efficient solvent for commercial use.

References

H. Wang, Y. Chen, L. Wang, Q. Liu, S. Yang, and C. Wang, “Advancing herbal medicine: enhancing product quality and safety through robust quality control practices,” Front Pharmacol, vol. 14, p. 1265178, 2023.

S. S. Belokurov, I. A. Narkevich, E. V Flisyuk, I. E. Kaukhova, and M. V Aroyan, “Modern extraction methods for medicinal plant raw material,” Pharm Chem J, vol. 53, no. 6, pp. 559–563, 2019.

J. Lim, K. Kim, D. Y. Kwon, J. K. Kim, R. Sathasivam, and S. U. Park, “Effects of Different Solvents on the Extraction of Phenolic and Flavonoid Compounds, and Antioxidant Activities, in Scutellaria baicalensis Hairy Roots,” Horticulturae, vol. 10, no. 2, p. 160, 2024.

M. J. Jara-Palacios, S. Gonçalves, F. J. Heredia, D. Hernanz, and A. Romano, “Extraction of antioxidants from winemaking byproducts: Effect of the solvent on phenolic composition, antioxidant and anti-cholinesterase activities, and electrochemical behaviour,” Antioxidants, vol. 9, no. 8, p. 675, 2020.

I. El Mannoubi, “Impact of different solvents on extraction yield, phenolic composition, in vitro antioxidant and antibacterial activities of deseeded Opuntia stricta fruit,” Journal of Umm Al-Qura University for Applied Sciences, vol. 9, no. 2, pp. 176–184, 2023.

H. Chen, H. Xiao, and J. Pang, “Parameter optimization and potential bioactivity evaluation of a betulin extract from white birch bark,” Plants, vol. 9, no. 3, p. 392, 2020.

A. R. Hakim and R. Saputri, “Narrative Review: Optimasi Etanol sebagai Pelarut Senyawa Flavonoid dan Fenolik: Narrative Review: Optimization of Ethanol as a Solvent for Flavonoids and Phenolic Compounds,” Jurnal Surya Medika (JSM), vol. 6, no. 1, pp. 177–180, 2020.

M. C. Dias, D. C. G. A. Pinto, and A. M. S. Silva, “Plant flavonoids: Chemical characteristics and biological activity,” Molecules, vol. 26, no. 17, p. 5377, 2021.

A. Tripathi, N. Chauhan, and P. Mukhopadhyay, “Recent advances in understanding the regulation of plant secondary metabolite biosynthesis by ethylene-mediated pathways,” Physiology and Molecular Biology of Plants, vol. 30, no. 4, pp. 543–557, 2024.

Kementerian Kesehatan RI, “Farmakope Herbal Indonesia (Edisi II),” Direktorat Jendereal Kementerian Kesehatan RI, 2017.

R. Fusco et al., “The role of cashew (Anacardium occidentale L.) nuts on an experimental model of painful degenerative joint disease,” Antioxidants, vol. 9, no. 6, p. 511, 2020.

C. Tshiama, D. Tshilanda, D. Tshibangu, N. Ngombe, T. Mbemba, and P. Mpiana, “antidiabetic, antisickling and antibacterial activities of Anacardium occidentale L.(Anacardiaceae) and Zanthoxylum rubescens Planch. Ex Hook (Rutaceae) from DRC,” Int J Diabet Endocrinol, vol. 3, pp. 7–14, 2018.

R. Amarowicz et al., “Free radical-scavenging capacity, antioxidant activity, and phenolic composition of green lentil (Lens culinaris),” Food Chem, vol. 121, no. 3, pp. 705–711, 2010.

R. L. Prior, X. Wu, and K. Schaich, “Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements,” J Agric Food Chem, vol. 53, no. 10, pp. 4290–4302, 2005.

R. Apak et al., “Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay,” Molecules, vol. 12, no. 7, pp. 1496–1547, 2007.

J.-K. Moon and T. Shibamoto, “Antioxidant assays for plant and food components,” J Agric Food Chem, vol. 57, no. 5, pp. 1655–1666, 2009.

B. Halliwell and J. M. C. Gutteridge, Free radicals in biology and medicine. Oxford university press, USA, 2015.

J. B. Harborne, “Metode Fitokimia, penuntun dan cara modern menganalisis Tumbuhan,” Penerjemah: Padmawinata K dan Soediro I. Penerbit ITB Bandung, 1996.

M. Zugazua-Ganado, A. Bordagaray, J. Ezenarro, R. Garcia-Arrona, M. Ostra, and M. Vidal, “Adaptation of the Folin-Ciocalteu and Fast Blue BB spectrophotometric methods to digital image analysis for the determination of total phenolic content: Reduction of reaction time, interferences and sample analysis,” LWT, vol. 193, p. 115756, 2024.

L. T. T. Nguyen, T. T. Nguyen, H. N. Nguyen, and Q. T. P. Bui, “Simultaneous determination of active compounds in Piper betle Linn. leaf extract and effect of extracting solvents on bioactivity,” Engineering Reports, vol. 2, no. 10, p. e12246, 2020.

S. Aryal, M. K. Baniya, K. Danekhu, P. Kunwar, R. Gurung, and N. Koirala, “Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal,” Plants, vol. 8, no. 4, p. 96, 2019.

Q. D. Do et al., “Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica,” J Food Drug Anal, vol. 22, no. 3, pp. 296–302, 2014.

S. Sai-Ut, P. Kingwascharapong, M. A. R. Mazumder, and S. Rawdkuen, “Optimization of ethanolic extraction of phenolic antioxidants from lychee and longan seeds using response surface methodology,” Foods, vol. 12, no. 15, p. 2827, 2023.

P. Sezer Okur and I. Okur, “Recent Advances in the Extraction of Phenolic Compounds from Food Wastes by Emerging Technologies,” Food Bioproc Tech, pp. 1–22, 2024.

A. Biswas et al., “Ultrasound-assisted extraction (UAE) of antioxidant phenolics from Corchorus olitorius leaves: a response surface optimization,” Chemical and Biological Technologies in Agriculture, vol. 10, no. 1, p. 64, 2023.

H. Lohvina, M. Sándor, and M. Wink, “Effect of Ethanol Solvents on Total Phenolic Content and Antioxidant Properties of Seed Extracts of Fenugreek (Trigonella foenum-graecum L.) varieties and determination of phenolic Composition by HPLC-ESI-MS,” Diversity (Basel), vol. 14, no. 1, p. 7, 2021.

N. Phuyal, P. K. Jha, P. P. Raturi, and S. Rajbhandary, “Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC,” The Scientific World Journal, vol. 2020, no. 1, p. 8780704, 2020.

J. Wairata, A. Fadlan, A. S. Purnomo, M. Taher, and T. Ersam, “Total phenolic and flavonoid contents, antioxidant, antidiabetic and antiplasmodial activities of Garcinia forbesii King: A correlation study,” Arabian Journal of Chemistry, vol. 15, no. 2, p. 103541, 2022.

C. Alasalvar, M. Karamać, R. Amarowicz, and F. Shahidi, “Antioxidant and antiradical activities in extracts of hazelnut kernel (Corylus avellana L.) and hazelnut green leafy cover,” J Agric Food Chem, vol. 54, no. 13, pp. 4826–4832, 2006.

R. Amarowicz, A. Troszynska, N. Baryłko‐Pikielna, and F. Shahidi, “Polyphenolics extracts from legume seeds: correlations between total antioxidant activity, total phenolics content, tannins content and astringency,” Journal of Food Lipids, vol. 11, no. 4, pp. 278–286, 2004.

R. Amarowicz et al., “Free radical-scavenging capacity, antioxidant activity, and phenolic composition of green lentil (Lens culinaris),” Food Chem, vol. 121, no. 3, pp. 705–711, 2010.

A. M. Shraim, T. A. Ahmed, M. M. Rahman, and Y. M. Hijji, “Determination of total flavonoid content by aluminum chloride assay: A critical evaluation,” Lwt, vol. 150, p. 111932, 2021.

J. Bliumaitė, A.-S. Maruška, D. Armonavičius, M. Stankevičius, A. Kanopka, and O. Ragažinskienė, “Evaluation of total phenolic content, total flavonoid content and radical scavenging activity in fractions of Epilobium angustifolium L. extracts and their in vitro anticancer activity,” in 18 th international scientific conference" The vital nature sign", May 16 th-17 th, 2024 Kaunas, Lithuania: abstract book/editors A. Maruška, N. Tiso, V. Kaškonienė, M. Stankevičius, Kaunas: Vytauto Didžiojo universitetas, 2024, p. 57.

M. O. Nguru, S. S. Gyang, C. J. Joseph, W. M. Iorjiim, S. Omale, and B. G. David, “Estimation of Total Phenolic and Total Flavonoid Contents, In-Vitro Antioxidant Activity and Median Inhibitory Concentration of Methanol Root Extract of Ximenia americana, L. Olacaceae,” J Adv Biol Biotechnol, vol. 24, no. 12, pp. 52–59, 2021.

M. Amiri, M. Arab, E. K. Sadrabad, N. Mollakhalili-Meybodi, and H. Fallahzadeh, “Effect of gamma irradiation treatment on the antioxidant activity, phenolic compounds and flavonoid content of common buckwheat,” Radiation Physics and Chemistry, vol. 212, p. 111127, 2023.

D. Huang, B. Ou, and R. L. Prior, “The chemistry behind antioxidant capacity assays,” J Agric Food Chem, vol. 53, no. 6, pp. 1841–1856, 2005.

W. Brand-Williams, M.-E. Cuvelier, and C. Berset, “Use of a free radical method to evaluate antioxidant activity,” LWT-Food science and Technology, vol. 28, no. 1, pp. 25–30, 1995.

P. Molyneux, “The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity,” Songklanakarin J. sci. technol, vol. 26, no. 2, pp. 211–219, 2004.

H. D. T. Madhuranga and D. N. A. W. Samarakoon, “Advancing In vitro Antioxidant Activity Assessment: A Comprehensive Methodological Review and Improved Approaches for DPPH, FRAP and H2O2 Assays,” Journal of Natural & Ayurvedic Medicine, vol. 7, no. 4, pp. 1–7, Oct. 2023, doi: 10.23880/jonam-16000431.

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Published

2024-09-30

How to Cite

Kurniasari, R., Suzery, M., & Cahyono, B. (2024). Analysis of Total Phenolics, Flavonoids, and Antioxidant Activity of Cashew Leaf Extract (Anacardium occidentale L.) with Varying Ethanol Concentrations: Analisis Total Fenolik, Flavonoid, dan Aktivitas Antioksidan Ekstrak Daun Jambu Mete (Anacardium occidentale L.) dengan Variasi Konsentrasi Etanol. Jurnal Riset Kimia, 15(2), 116–130. https://doi.org/10.25077/jrk.v15i2.735

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Vol. 15 No. 2 (2024): September

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