Development of The Scanometric Method for Caffeine Determination in Pharmaceutical Preparations

Authors

  • Mochammad Amrun Hidayat Chemo and Biosensors Group, Faculty of Pharmacy, University of Jember, Kalimantan I/2, Kampus Tegalboto, Jember, East Java, Indonesia
  • Yunita Chemo and Biosensors Group, Faculty of Pharmacy, University of Jember, Kalimantan I/2, Kampus Tegalboto, Jember, East Java, Indonesia
  • Rahma Riska Panida Chemo and Biosensors Group, Faculty of Pharmacy, University of Jember, Kalimantan I/2, Kampus Tegalboto, Jember, East Java, Indonesia
  • Indah Yulia Ningsih Chemo and Biosensors Group, Faculty of Pharmacy, University of Jember, Kalimantan I/2, Kampus Tegalboto, Jember, East Java, Indonesia
  • Bambang Kuswandi Chemo and Biosensors Group, Faculty of Pharmacy, University of Jember, Kalimantan I/2, Kampus Tegalboto, Jember, East Java, Indonesia

DOI:

https://doi.org/10.25077/jrk.v16i1.756

Keywords:

NaIO4, CH3COOH, MBTH, caffeine, scanometric method

Abstract

The caffeine assay can be developed by using sensitive reagents for caffeine detection, such as sodium periodate (NaIO4), acetic acid (CH3COOH), and 3-methyl-2-benzothiazolinone hydrazone (MBTH) inside 96-microwell plate. The addition of caffeine solution could change the color of the reagent from white to deep blue which can be then scanned and quantified by a flatbed scanner and the color image analysis program, known as the scanometric method. Analytical characterization such as linearity, LOD, LOQ, precision, accuracy, and stability of the developed method was established by using caffeine standard. The result of caffeine analysis using the developed method agreed with that of the spectrophotometric method, suggesting that the developed method can be used as an alternative method for caffeine assay in pharmaceutical preparations.

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Published

2025-03-31

How to Cite

Hidayat, M. A., Yunita, Panida, R. R., Ningsih, I. Y., & Kuswandi, B. (2025). Development of The Scanometric Method for Caffeine Determination in Pharmaceutical Preparations. Jurnal Riset Kimia, 16(1), 113–120. https://doi.org/10.25077/jrk.v16i1.756

Issue

Vol. 16 No. 1 (2025): March

Section

Articles

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