Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya

Authors

  • Cori Yolanda Elise Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Sumatra Barat, Indonesia
  • Syafrizayanti Syafrizayanti Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Sumatra Barat, Indonesia https://orcid.org/0000-0002-4896-2722
  • Marniati Salim Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, Sumatra Barat, Indonesia

DOI:

https://doi.org/10.25077/jrk.v12i2.412

Keywords:

phycocyanin, Spirulina platensis, liquid biphasic flotation (LBF), DPPH, IC

Abstract

Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of   338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.

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Published

2021-09-09

How to Cite

Elise, C. Y., Syafrizayanti, S., & Salim, M. (2021). Pemurnian Fikosianin dari Spirulina platensis dengan Metode Liquid Biphasic Flotation (LBF) dan Penentuan Aktivitas Antioksidannya. Jurnal Riset Kimia, 12(2). https://doi.org/10.25077/jrk.v12i2.412

Issue

Vol. 12 No. 2 (2021): September

Section

Articles

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